中国药理学通报2011，Vol.27Issue(2)：229-233,5.
N-乙酰半胱氨酸影响p38有丝分裂原活化蛋白激酶对顺铂诱导急性肾损伤的保护作用
Protective effect of N-acetylcysteine on cisplatin-induced acute Kidney injury related to p38MAPK pathway in rats
摘要
Abstract
Aim To investigate the effects of N-acetylcysteine( NAC ) on cisplatin ( CDDP )-induced acute kidney injury( AKI ) and its relationship with p38 mitogen activated protein kinase( p38 MAPK ) pathway.Methods The rat was injected CDDP intravenously to make AKI model.The rats were randomly divided into 6 groups,including the normal control group, AKI model group, NAC 50 mg · kg-1 group, NAC 100 mg·kg-1 group,NAC 200 mg · kg-1 group and p38MAPK specific inhibitor SB203580 10 mg· kg-1 group.NAC and SB203580 were administered once a day for three days before CDDP was given.And then NAC and SB203580 were continuously given o.d.for five days.The apoptosis of kidney was monitored by TUNEL.The content of caspase-3 in kidney was determined by the commercial kit.The expression of caspase-3 , Bax , Bcl2 , phosphorylated p38MAPK( p-p38MAPK )were measured by Western blot.Results Compared with the normal control group , apoptosis cells and expressions of caspase-3、Bax、p-p38MAPK in kidney were increased and the expression of Bcl-2 in kidney was decreased( P ＜0.01 )in the model group.NAC and SB203580 reduecd the apoptosis cells, supressed the expressions of caspase-3 , Bax, p-p38MAPK and augmented the expression of Bcl-2 to protect kidney from CDDP injury compared with the AKI model group( P＜0.01 ).Conclusions NAC may prevent kidney from CDDP impairment via the p38 MAPK pathway.关键词
N-乙酰半胱氨酸/p38有丝分裂原活化蛋白激酶/顺铂/急性肾损伤/凋亡/大鼠分类
医药卫生引用本文复制引用
罗景慧,杨迎暴,安田日出夫,菱田明..N-乙酰半胱氨酸影响p38有丝分裂原活化蛋白激酶对顺铂诱导急性肾损伤的保护作用[J].中国药理学通报,2011,27(2):229-233,5.基金项目
广东省医学科研基金资助项目(No A2008413) （No A2008413）
南方医科大学南方医院院长基金资助项目(No 2009B029) （No 2009B029）
