中国药理学通报2011，Vol.27Issue(2)：248-252,5.
脊髓c-Jun在NMDA受体NR2B亚基介导的大鼠吗啡镇痛耐受中的作用
Role of c-Jun of spinal cord in NMDA receptor subunit NR2B mediated morphine antinociceptive tolerance in rats
摘要
Abstract
Aim To investigate the role of spinal cord c-Jun in the development of N-methyl-D-aspartate ( NMDA )receptor subunit NR2B mediated morphine antinociceptive tolerance.Methods Morphine 10 μl ( 1.5 g · L-1 ) was administered intrathecally for consecutive 7 days to achieve analgesic tolerance to morphine.Hot water tail flick test was used to assess the analgesic efficacy.Immunohistochemistry assay was applied to detect the expressions of phosphorylated cJun( p-c-Jun ) and total c-Jun( t-c-Jun ).Results At the 7th day of repeated intrathecal injection of morphine, c-Jun was activated remarkably in the lumbar spinal cord.which mainly existed in neurons.Repeated intrathecal injection of the NR2B competitive inhibitor Ro256981 10 μl( 2 g · L-1 )coadministrated with morphine not only partly inhibited the activation of c-Jun induced by chronic morphine treatment.but also markedly attenuated the development of morphine antinociceptive tolerance.Conclusion The spinal cord neuron p-c-Jun contributes to NMDA receptor subunit NR2B mediated morphine antinociceptive tolerance.关键词
吗啡镇痛耐受/神经元/N-甲基-D-天冬氨酸受体/NR2B亚基/c-Jun/JNK分类
医药卫生引用本文复制引用
傅璐,郭瑞鲜,莫利求,崔宇,赵春梅,胡芬,陈培熹,冯鉴强..脊髓c-Jun在NMDA受体NR2B亚基介导的大鼠吗啡镇痛耐受中的作用[J].中国药理学通报,2011,27(2):248-252,5.基金项目
广东省科技计划资助项目(No 2010B080701035,2008B080703053) （No 2010B080701035,2008B080703053）
