中国药理学通报2011，Vol.27Issue(2)：196-200,5.
鞘内注射螺内酯对大鼠神经根痛的影响
Effects of intrathecal injection of spironolactone on a model of radicular pain in rats
摘要
Abstract
Aim To investigate the effects of intrathecal delivery of spironolactone on radicular pain behaviors and the underlying mechanisms.Methods Using rat model of radicular pain induced by chronic compression of dorsal root ganglion( CCD ).77 male SD rats successfully received intrathecal catheter implantation and without motor dysfunction were randomly divided into 3 groups: sham-operation group ( Sham group, n = 27 ) , chronic compression of dorsal root ganglion group ( CCD group , n = 2 7 ) and spironolactone group( Spir group,n =23 ).Rats in Spir group were intrathecally treated with spironolactone 3 μg/10 μl twice daily on day 2 -4 after CCD,while rats in CCD and Sham group received 10 μl 10％alcohol.Paw withdrawal mechanical threshold ( PWMT ) and paw withdrawal thermal latency( PWTL )were tested on day 1 before CCD and day 1,4,7,10 ,14 after CCD( n = 12 in CCD and Sham group ,n =8 in Spir group ).The expression of phosphorylated p65( p-p65 )among 3 groups on day 4,7 ,10 after CCD was revealed by Western blot ( n =5 in each group ).Results Compared with Sham group, both PWMT( P＜0.01 )and PWTL( P＜0.01)were significantly decreased after CCD on the ipsilateral side , so were the expression of p-p65( P ＜ 0.01 ).No significant difference was found in sham group( P ＞0.05 )and on the contralateral side( P ＞ 0.05 ).Intrathecal spironolactone significantly reduced mechanical allodynia( 11.58±1.38 v.s 7.71 ± 0.82 on Day 4,P ＜0.01;10.89 ± 1.46 v.s 7.49 ± 1.11 on Day 7,P ＜0.01 )and thermal hyperalgesia( 14.14 ±1.52 vs 8.19 ±1.32 on Day 4,P ＜ 0.01 ; 13.56± 1.63 vs 8.56 ±1.56 on Day 7,P ＜ 0.01 )in comparison to vehicletreated CCD rats.Consistently, the expression of p-p65 also significantly decreased( P ＜0.01 ).Conclusions Intrathecal spironolactone had therapeutic effects on radicular pain induced by chronic compression of dorsal root ganglion in rats.The inhibition of MR-NF-KB pathway was one of the main underlying mechanisms.关键词
神经病理性疼痛/中枢敏化/盐皮质激素受体/螺内酯/核转录因子κB/促炎介质分类
医药卫生引用本文复制引用
彭良玉,顾小萍,马擎,朱蓓蓓,宋丽华,孙晓凤,马正良..鞘内注射螺内酯对大鼠神经根痛的影响[J].中国药理学通报,2011,27(2):196-200,5.基金项目
国家自然科学基金资助项目(No 30872439/c16060202) （No 30872439/c16060202）
