中国全科医学2011，Vol.14Issue(3)：285-287,3.
银杏叶提取物对2型糖尿病大鼠心肌细胞钙通道基因表达的影响研究
Ginkgo Biloba Extracts on the Calcium Channel Gene Expression in Cardiomyocyte of the Experimental Type 2 Diabetes Rats
摘要
Abstract
Objective To investigate the influence of Ginkgo biloba extracts on the L-type calcium channel protein mRNA expression of the cardiomyocyte in the experimental type 2 diabetes rats, so as to study the therapeutic effect of ginkgo biloba extracts on the type 2 diabetes. Methods Diabetes rat model were induced by high glucose and high fat feedstuff and low-dosage streptozotocin. The 20 rats were randomized into diabetes group, therapy group, and 10 additional normal rates were taken as control group. The rats in therapy group were lavaged with ginkgo biloba extracts, the other two groups were lavaged with 0. 9％ NaCl injection. After ten weeks, mRNA expressions of the L - type calcium channel protein of all groups were detected by RT-PCR. Results The expressions of the L- type Calcium protein mRNA in each group were ( 0. 157 ± 0. 028 ), ( 0. 257 ± 0. 025 ), and ( 0. 226 ± 0. 029 ) respectively, the differences were significant ( F = 28. 232, P = 0. 000 ). The expressions of the L -type Calcium protein mRNA of diabetes group and therapy group were significantly higher than that of the control group, while the therapy group was significantly lower than the model group. ( P ＜0. 05 ). After 10 weeks, the level of blood glucose in diabetes group was significantly increased compared with control group, while it is significantly decreased in the therapy group compared with diabetes group ( P ＜ 0. 05 ). Conclusion Ginkgo biloba extracts can decrease the blood glucose and the L-type calcium channel protein mRNA expression in the cardiomyocyte of the type 2 diabetes rats. Ginkgo biloba extracts have therapeutic effect on type 2 diabetes.关键词
糖尿病,2型/银杏叶提取物/钙通道/L型分类
医药卫生引用本文复制引用
念红,宋宝辉,吴红,刘桂莲,孙革..银杏叶提取物对2型糖尿病大鼠心肌细胞钙通道基因表达的影响研究[J].中国全科医学,2011,14(3):285-287,3.基金项目
2009年黑龙江省卫生厅科研项目--银杏叶提取物对2型糖尿病大鼠心肌保护机制的研究(2009-408) （2009-408）
2008年牡丹江医学院科研项目--银杏叶提取物对实验性2型糖尿病大鼠心肌细胞钙通道基因表达的影响研究(B200806) （B200806）
