中国全科医学2011，Vol.14Issue(3)：274-276,3.
溃疡性结肠炎和克罗恩病患者端粒结合蛋白TRF1和TRF2及RAP1mRNA表达的变化
Altered mRNA Expression of Telomere Binding Proteins(TRF1,TRF2 and RAP1) in Ulcerative Colitis and Crohn's Disease
董玉柱1
作者信息
- 1. 257055,山东省东营市胜利医院消化内科
- 折叠
摘要
Abstract
Objective To observe mRNA expression in telomere binding proteins ( TRF1, TRF2 and RAP1 ) in ulcerative colitis ( UC ) and Crohn's disease ( CD ) and analyze the relationship. Methods Peripheral blood mononuclear cells ( PBMC ) obtained from 34 IBD patients ( 18 ulcerative colitis and Crohn's disease ) and 14 controls were activated by phytohaem-agglutinin and purified to yield activated ( CD+25 ) T lymphocytes. TRF1, TRF2 and RAP1 mRNA expression in PBMC and activated T lymphocytes was measured by RT - PCR. Results TRF1 mRNA levels in PBMC and TRF2 mRNA levels in T lymphocytes were significantly reduced in both UC and CD subjects compared with the control group ( P ＜0. 05 ). Levels of RAP1 mRNA in T lymphocytes were significantly lower in UC subjects compared with the control group ( P ＜0. 01 ), but not in CD subjects ( P ＞ 0. 05 ). Levels of TRF1 mRNA in PBMC of UC subjects were significantly increased compared with CD subjects ( P ＜ 0. 01 ). Conclusion The down regulation of TRF2 and RAP1 mRNA expression in CD+25 T - lymphocytes in IBD suggests that these telomere binding proteins play a role in telomere regulation and may contribute to the telomeric fusions and chromosomal abnormalities observed in UC. These findings may also indicate a systemic process of telomere uncapping which could represent a biomarker for IBD associated cancer risk.关键词
炎性肠病/溃疡性结肠炎/克罗恩病/端粒结合蛋白/mRNA分类
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董玉柱..溃疡性结肠炎和克罗恩病患者端粒结合蛋白TRF1和TRF2及RAP1mRNA表达的变化[J].中国全科医学,2011,14(3):274-276,3.
