中国药理学通报2011，Vol.27Issue(1)：103-106,4.
2-甲氧基雌二醇对CEM细胞增殖的影响及其机制研究
Effects of 2-methoxyestradiol on CEM cells and its underlying mechanisms
摘要
Abstract
Aim To study the effect of 2-methoxyestradiol( 2-ME2 )on human leukemia CEM cells and its underlying mechanisms.Methods CEM cells were treated with different concentrations of 2-ME2 at 48 h.MTT assay was used to examine the effect of growth inhibition.CEM cells was treated with 2 μmol· L-1 2ME2 at 0.24, 48 and 72 h.The expression of VEGF and hTERT mRNA were detected by RT-PCR; the expression of Akt and p-Akt proteins were detected by Western blot.Results Different concentrations of 2ME2 inhibited proliferation of CEM cells in a dose-dependent manner with the ICso was 2 μmol· L-1;2ME2 decreased the expression of VEGF and hTERT mRNA, and down-regulated proteins level of p-Akt,while it had no effect of total Akt protein in CEM cells after treated with 2 μmol· L-1 of 2-ME2 for 0.24,48 and 72 h.Conclusions 2-ME2 decreases the expression of hTERT and interferes in PI3K/Akt signaling pathway through partly decreasing the expression of VEGF mRNA , which provides a possible mechanism for explaining the 2-ME2' s antiproliferative activity.关键词
2-甲氧基雌二醇/白血病/CEM细胞/Akt/hTERT/VEGF分类
医药卫生引用本文复制引用
张学亚,潘敬新,郭熙哲,战榕..2-甲氧基雌二醇对CEM细胞增殖的影响及其机制研究[J].中国药理学通报,2011,27(1):103-106,4.基金项目
福建省卫生厅青年课题基金资助(No 2006-1-38) （No 2006-1-38）
福建省科技厅资助省属高校项目(No 2006F5041) （No 2006F5041）
