中国药理学通报2011，Vol.27Issue(1)：77-81,5.
内吗啡肽在小鼠树突状细胞的诱导表达
Inducible expression of endomorphins in murine dendritic cells
摘要
Abstract
Aim To study the expression of endomorphin( EM )-1 and EM-2 in dendritic cells( DCs ).Methods Bone marrow dendritic cells were extracted from 7- to 8-week-old C57BL/6J mice, purified with anti-CDllc( a specific marker for dendritic cells ) antibody-coated magnetic beads and cultured in the presence of GM-CSF.FACS analysis indicated that the purity of DCs was more than 95％.Immunofluorescence staining was used to detect to expression of EMs in DCs.The enzyme immunoassay( EIA ) was used to measure the contents of EMs.Results Immunofluorescence staining revealed that EM-1 and EM-2 were expressed and upregulated in DCs activated by LPS.Enzyme immunoassay showed that LPS and other Toll-like receptor( TLR )ligands promoted the secretion of EM-1 and EM-2 from activated DCs.Conclusions LPS can activate DCs and upregulate the expression of EM-1 and EM-2.TLR ligands can promote the secretion of EMs from DCs.关键词
内吗啡肽-1/内吗啡肽-2/Mu阿片受体/树突状细胞/抗原递呈细胞/Toll样受体/酶免疫测定分类
医药卫生引用本文复制引用
李正红,高琴,叶红伟,单立冬,龚珊,蒋星红..内吗啡肽在小鼠树突状细胞的诱导表达[J].中国药理学通报,2011,27(1):77-81,5.基金项目
安徽省自然科学基金资助项目(No 090413097) （No 090413097）
苏州大学中日校际合作研究资助项目(No EE 134005) （No EE 134005）
