中国药理学通报2011，Vol.27Issue(1)：95-98,4.
NAC拮抗As2O3对外周血淋巴细胞的毒性作用
Antagonistic effects of N acetylcysteine on the cytotoxicity of arsenic trioxide to lymphocytes
摘要
Abstract
Aim To study the cytotoxicity of arsenic trioxide ( As2O3 ) to lymphocytes and the antagonistic and protective effects of N acetylcysteine ( NAC ).Methods Lymphocytes were separated from normal human peripheral blood.The cell survival was examined by Trypan blue dye exclusion assay , apoptosis of lymphocytes was detected by electron microscopy and double staining of FITC-Annexin V/PI, and the cellular reactive oxygen species( ROS )level and Bcl-2 expression were analyzed by flow cytometry( FCM ).Results As2O3 markedly inhibited the growth of human lymphocytes, and induced the cells to display the typical apoptotic changes.During As2O3-induced apoptosis of lymphocytes , the expression of Bcl-2 protein decreased and the production of ROS in the As2O3-treated lymphocytes increased.Compared with As2O3-treatment alone , the combined action of NAC and As2O3 markedly repressed the As203-induced proliferation inhibition and apoptosis of lymphocytes, meanwhile.the production of ROS reduced and the expression of Bcl-2 protein was raised in the lymphocytes.Conclusion As2O3 exerts a significant cytotoxicity to human lymphocytes , and NAC antagonizes the As2O3 -induced cytotoxicity of lymphocytes via elimination of ROS and up-regulation of Bcl-2 expression.关键词
N-乙酰-L-半胱氨酸(NAC)/三氧化二砷(AS2O3)/淋巴细胞/细胞凋亡/活性氧(ROS)/Bcl-2/细胞毒性分类
医药卫生引用本文复制引用
李彩丽,魏虎来,苏海翔..NAC拮抗As2O3对外周血淋巴细胞的毒性作用[J].中国药理学通报,2011,27(1):95-98,4.基金项目
甘肃省科技攻关资助项目(No GS022-A43-137) （No GS022-A43-137）
