中国药理学通报2011，Vol.27Issue(1)：117-121,5.
Na+,K+-ATP酶参与大鼠皮层神经元NMDA电流的调节
Comprehensive study of N-methyl-D-aspartate current involving Na+, K+-ATPase in rat cortical neuron
摘要
Abstract
Aim To explore under normoxia and hypoxia conditions whether the Na+.K+-ATPase was involved in the change of the excitatory amino acid current in rat cortical neuron and its mechanism.Methods The whole-cell mode was used to examine NMDA current in rat brain slices by the patch clamp systems with infrared differential interference contrast ( IRDIC ) optics and to observe the effects of different concentrations of dihydroouabain ( DHO ) on NMDA current.Results Under either normoxia or hypoxia conditions.DHO and vanadate decreased the NMDA current in a concentration-dependent manner.However,NMDA current densities decreased by different concentrations of DHO were much larger under hypoxia than those under normoxia.Conclusion The regulation of NMDA current of cortical neuron after hypoxia may depend mainly on the release of excitatory amino acid.not the function of the Na+ , K+ -ATPase.关键词
Na+,K+-ATP酶/NMDA/缺氧/神经元/膜片钳/脑片分类
医药卫生引用本文复制引用
刘倩,郭会彩,张丽男,王永利..Na+,K+-ATP酶参与大鼠皮层神经元NMDA电流的调节[J].中国药理学通报,2011,27(1):117-121,5.基金项目
河北省自然科学基金资助项目(No 301360) （No 301360）
河北省教育厅资助项目(No 2010148) （No 2010148）
