中国药理学通报2011，Vol.27Issue(1)：85-90,6.
黄芪注射液对缺氧缺糖/复氧复糖大鼠海马神经元caspase-3表达的作用
The effect of astragalus injection on the expression of caspase-3 after hypoxia/hypoglycemia and reoxygenation in hippocampal neurons of rats
摘要
Abstract
Aim To investigate the effect of astragalus injection on the expression of caspase-3 interrelated by apoptosis after hypoxia/hypoglycemia and reoxygenation in hippocampal neurons of rats.Method The hippocampal neurons cultured for eight days were divided into four groups : normal control group( normal ) , hypoxia/hypoglycemia and reoxygenation group( model ).astragalus injection solution group( solution ), astragalus injection group( astragalus ).All groups except normal group were treated with hypoglycemia and reoxygenation after deprived of oxygen and glucose for 30 minutes.Methods of Immunohistochemistry and Western blot were used to measure the expression of caspase-3 protein.In situ hubridization was used to measure the expression of caspase-3 mRNA after hypoxia/hypoglycemia and reoxygenation 0 h,0.5 h.2 h,6 h,24 h,48 h,72 h,120 h.Results The results of immunohistochemistry were similar to those of in situ hybridization.Compared with normal group, the numbers, the percentage of caspase-3 protein positive neuronal cells at every time point increased obvious in the model group except 0 h and 0.5 h( P ＜0.05 ).and the peak arrived at 24 h; the change of neuronal configuration.the numbers.the percentage of caspase-3 protein positive neuronal cells in solution group accorded with those in model group( P＞ 0.05 ); compared with model group, the numbers, the percentage of caspase-3 protein were less obvious in the astragalus injection group except 0 h and 0.5 h( P＜ 0.05 ).Western blot results showed that:compared with normal group , the expression of caspase3 protein in hippocampal neurons of rats at every time point increased obviously in model group except Oh and 0.5 h( P ＜ 0.05 ), and peaked at 24 h; compared with model group, the expression of caspase-3 protein at every time point in astragalus injection solution group had no obvious change( P＞ 0.05 ), however that in hippocampal neurons of rats at every time point decreased obviously in the astragalus injection group except 0 h and 0.5 h( P＜0.05 ).Conclusion Astragalus injection can inhibit the expression of caspase-3 after hypoxia/hypoglycemia and reoxygenation , and accordingly inhibits hippocampal neuronal apoptosis.关键词
海马神经元/黄芪注射液/缺氧缺糖/复氧复糖/细胞凋亡/半胱氨酸天冬氨酸蛋白酶3/作用机制分类
医药卫生引用本文复制引用
闫凤霞,高维娟,钱涛,叶冬青,任立群,张雅丽,侯志平..黄芪注射液对缺氧缺糖/复氧复糖大鼠海马神经元caspase-3表达的作用[J].中国药理学通报,2011,27(1):85-90,6.基金项目
教育部新世纪优秀人才支持计划(No NCET-06-0258) （No NCET-06-0258）
河北省科技领军人才创新基金(No 06547009D-11) （No 06547009D-11）
河北省自然科学基金资助项目(No C2006000865) （No C2006000865）
河北省教育厅科学研究计划课题(No 2005224) （No 2005224）
河北省科技支撑计划(No 201021025) （No 201021025）
