安徽中医学院学报2011，Vol.30Issue(1)：53-56,4.DOI:10.3969/j.issn.1000-2219.2011.01.020
新藤黄酸体外抑制肺腺癌A549细胞增殖的作用
Inhibitive Effects of Gambogenic Acid on Proliferation of Lung Adenocarcinoma A549 Cells In Vitro
摘要
Abstract
Objective To investigate anti-proliferation effect of Gambogenic acid (GNA) on lung adenocar cinoma A549 cells and its underlying mechanisms. Methods A549 cells were treated with different concen tration of GNA for 24, 48 and 72 h, respectively；the inhibitive effect was measured by methyl thiazolyl tetrazolium (MTT) assay;alternation of ultra structure was detected by AO/EB staining under fluorescent microscope ； lactate dehydrogenase (LDH) assay was applied to determine whether GNA induced A549 cells necrosis. Results MTT assay evidenced that GNA inhibited A549 cells proliferation in a dosage dependent manner within the concentration ranging from 0 to 8 μmol/L and time-dependent manner from 24 to 72 h; AO/EB staining under fluorescent microscope showed that 24 h GNA treatment could induce typical apop tosis in A549 cells； LDH assay showed that no significant difference in the LDH release was observed after GNA treatment for 24 h in the concentration ranging from l to 4μmol/L, while 8 μmol/L GNA could sta tistically induce LDH release in comparison with the control group (P＜0.01). Conclusion GNA has the capacity to inhibit the proliferation of human lung adenocarcinoma A549 cells. Its underlying mechanisms is related to apoptosis inducing.关键词
新藤黄酸/肺腺癌A549细胞/细胞凋亡分类
医药卫生引用本文复制引用
朱国旗,程卉,王训翠,王效山,李庆林..新藤黄酸体外抑制肺腺癌A549细胞增殖的作用[J].安徽中医学院学报,2011,30(1):53-56,4.基金项目
安徽省高校自然科学研究项目(KJ2009B197Z) （KJ2009B197Z）
国家重大新药创制专项(2009ZX09103-399) （2009ZX09103-399）
