神经损伤与功能重建2011，Vol.6Issue(1)：19-23,5.DOI:10.3870/sjsscj.2011.01.005
pEGFP/A2M(FP6)重组质粒转染小鼠胚胎神经干细胞的实验研究
Transfection of pEGFP/A2 M (FP6) Plasmid Vectors into Mouse Embryonic Neural Stem Cells
武强 1李露斯 2范文辉 2黎红华 1崔敏 1吴非 1徐志鹏 1濮捷 1杨梅1
作者信息
- 1. 广州军区武汉总医院神经内科,武汉,430070
- 2. 第三军医大学附属西南医院神经内科,重庆,400038
- 折叠
摘要
Abstract
Objective: To study the biological features of the mouse embryonic neural stem cells (NSCs) transfected by pEGFP/A2M(FP6). Methods: NSCs of mouse embryos were isolated and cultured. The pEGFP/A2M (FP6) plasmid vectors were transferred into the cultured NSCs by Nucleofector TM. The expression of A2M FP6 mRNA was detected with RT-PCR and immunofluorenscent analysis. Results: The pEGFP/A2M(FP6) plasmid vectors were successfully transferred into NSCs in which expression of A2M(FP6) remained rich. In addition, immunohischemistry showed that the proliferation and differentiation of NSCs were unchanged. Conclusion: The proliferation and differentiation of NSCs after tansfection with pEGFP/A2M (FP6)were unaltered, keeping the ability to produce A2 M(FP6).关键词
pEGFP/A2M(FP6)/Nucleofector转染/神经干细胞Key words
pEGFP/A2M(FP6)/ Nucleofector transfection/ neural stem cells分类
医药卫生引用本文复制引用
武强,李露斯,范文辉,黎红华,崔敏,吴非,徐志鹏,濮捷,杨梅..pEGFP/A2M(FP6)重组质粒转染小鼠胚胎神经干细胞的实验研究[J].神经损伤与功能重建,2011,6(1):19-23,5.
