重庆医学2011，Vol.40Issue(1)：53-54,封3,3.DOI:10.3969/j.issn.1671-8348.2011.01.023
25例慢性心力衰竭患者炎症细胞因子的变化
Relationship between the secretion of cytokines and the activation of NF-κB in peripheral mononuclear cells from 25 patients with chronic heart failure
刘家勉 1刘应才2
作者信息
- 1. 四川省乐山市人民医院心内科,614000
- 2. 泸州医学院附属医院心内科,四川,646000
- 折叠
摘要
Abstract
Objective To investigate the relationship between the secretion of cytokines and the activation of NF κB in peripheral mononuclear cells(PBMCs) from patients with chronic heart failure(CHF). Explore the possible mechanism of CHF. Methods PBMCs isolated from 25 CHF patients and 25 healthy subjects were stimulated by LPS in vitro for 24 hs. The supernatant fluid were used to measure the level of TNF-α, IL-1β, IL-6 by radioimmunoassay, and the activation of NF-κB in PBMCs were measured by immunohistochemical method. Results The secretion of cytokines(TNF-α,IL-lβ,IL-6) and the activation of NF-κB in PBMCs showed a significant difference between CHF and healthy(P＜0.05). The correlative analyses indicated that a positive correlation existed between the activation degree of NF-κB and the level of cytokines in PBMCs. Conclusion In patients with CHF, the secretion of cytokines may be regulated by NF-κB way. This property may be one of the mechanism for heart failure.关键词
心力衰竭,充血性/单核细胞/NF-κB/肿瘤坏死因子/白细胞介素6Key words
heart failure/ congestive/ monocytes / NF-kappa B/ tumor mecrosia factor/ interleukin 6引用本文复制引用
刘家勉,刘应才..25例慢性心力衰竭患者炎症细胞因子的变化[J].重庆医学,2011,40(1):53-54,封3,3.
