重庆医学2011，Vol.40Issue(1)：3-6,4.DOI:10.3969/j.issn.1671-8348.2011.01.002
3种神经病理性疼痛大鼠模型的评价
Difference of the characteristics of pain proceeding and the level of oxidative stress between the three neuropathic pain models
摘要
Abstract
Objective The models of peripheral spinal nerve injury can be classified as spared nerve injury model(SN1) ,chronic constriction injury of the sciatic nerve model(CCI),and improved chronic constriction injury of the sciatic nerve model(iCCI). To compare the principle and characteristic of pain proceeding as well as the level of oxidative stress after peripheral spinal nerve injury between the three models. Methods Sixty female SD rats(weighted from 150 to 200 g) were randomized into four groups (group Sham,group CCI,group iCCI,group SNI,n= 15). The sciatic nerve of the right hind leg was exposed without any injury in group Sham, but operated according to the methods described following in group CCI,group iCCI and group SNI. Then appraisaling the degree of neuropathic pain by the scores of 50 ％ paw withdraw thresholds and radiant heat stimulation before the surgery and 1,3, 7,14,17,21 days later. Thc activities of SOD and content of MDA were measured respectively in thc lumbosacral spinal cord 7,14, 21 days later after surgery. Results (1)During experiments, any sign of mechanical or heat allodynia did not occur in group Sham. But the surgical procedure in group CCI and iCCI produced an apparent and stable hyperalgesia to noxious heat and mechanical stimulation since the 7th day after operation,and reached the lowest peak on thc 14th day bothly. The degression of pain threshold was more responsive in group iCCI than group CCI after the 7th day of the surgery. The group SNI showed insensitive to heat stimulation but more sensitive to the mechanical stimulation than group CCI and iCC1. (2)There was a significant difference of the oxidative stress between the three models(P＜0.05). The activity of SOD dropped while the content of MDA raised in all the surgery group but group Sham. The change amplitude was group iCCI＞CCI＞SNI. Conclusion The principles of pain proceeding and level of MDA,SOD are different between the three modcls. This suggests that different peripheral spinal nerve injuries may have different influence in the mechanism of neuropathic pain and oxidative stress.关键词
疼痛/模型,动物/丙二醛/超氧化物歧化酶/外周神经损伤Key words
pain/models,animal/malondialdedyde/superoxide dismutase/peripheral spinal nerve injury引用本文复制引用
林露,陶国才,易斌..3种神经病理性疼痛大鼠模型的评价[J].重庆医学,2011,40(1):3-6,4.基金项目
重庆市自然科学基金资助项目(2007BB5063). （2007BB5063）
