中国免疫学杂志2011，Vol.27Issue(4)：303-307,5.DOI:10.3969/j.issn.1000-484X.2011.04.004
鞭毛蛋白与IL-12协同诱导人NK细胞产生IFN-γ机制的探讨
The synergistic effect of flagellin and interleukine-12 on the induction of IFN-γ production from human NK cells and its mechanism
摘要
Abstract
Objective :To study the synergic effect of flagellin and interleukine-12 (IL-12 ) on the production of IFN-γ by human NK cells and it_s mechanisnl .Methods :PBMCs isolated from normal donors were cultured with medium ,flagellin ,IL-12 or flagellin plus IL-12 .Culture supematants were collected at day 3 and the level of IFN-γ was measured by ELISA .Cells under primary culture conditions were collected , washed ,restimulated ,and the production of IFN-γ was detected after 3 days .The expression of IL-12 receptor β1 and toll-like receptor 5 on NK cell subsets was detected and analyzed by flow cytometry .Results :In the primary culture conditions ,application of suboptimal dose of flagellin or IL-12 could induce low levels of IFN-γ .Stimulation of PBMCs with flagellin plus IL-12 could synergistically induce IFN-γ production .Under the secondary culture conditions ,cells that pre-treated with flagellin were restimulated with different concentrations of IL-12 ,which could induce high levels of IFN-γ in a dose-dependent manner .Cells pre-stimulated with IL-12 could ,in a does-dependent manner ,enhance the production of IFN-γ in the presence of different concentrations of flagellin .Flow cytometry analysis indicated that the expression of IL-12 receptorβ1 on NK cell subsets was upregulatecl when stimulated with flagellin and IL-12 could enhance the expression of toll-like receptor 5 on NK cell subsets . Conclusion :Up-regulation of IL-12 receptorβ1 by flagellin and enhanced expression of the toll-like receptor 5 by IL-12 contribute to the svnergistic effects of IFN-γ production by NK cell subsets .关键词
鞭毛/IL-12/NK细胞/IFN-γ分类
医药卫生引用本文复制引用
李琴,刘昀,吴长有..鞭毛蛋白与IL-12协同诱导人NK细胞产生IFN-γ机制的探讨[J].中国免疫学杂志,2011,27(4):303-307,5.基金项目
本课题为教育部2007年度高等学校博士学科点专项科研基金(20070558213)项目 （20070558213）
