中国免疫学杂志2011，Vol.27Issue(4)：295-299,5.DOI:10.3969/j.issn.1000-484X.2011.04.002
信号分子IRAK1/4在anti-β2GPI/β2GPI复合物诱导THP-1细胞表达TF中的作用探讨
The roles of IRAK1/4 in anti-β2GPI/β2GPI-induced tissue factor expression in THP-1 cells
摘要
Abstract
Objective :To investigate the role of IRAK1 and IRAK4 in tissue factor expression of THP-1 cells treated with anti-β2GPI/ β2GPI complex .Methods :The TF expression of THP-1 cells was detected by Real-time PCR or TF activity kits after stimulating with antiβ2GPI/β2GPI complex .The IRAK1 ,β-IRAK1 and IRAK4 expression induced by anti-β2GPI/β2GPI complex was determined by Western blot . It was also investigated that IRAK1/4 inhibitor could interrupt the TF expression in THP-1 cells stimulated with anti-β2GPI/β2GPI complex . Results :The TF expression (both mRNA and activity ) of THP-1 cells could be significantly up-regulated with the treatment of anti-β2GPI/ β2GPI complex (100 μg/ml) .The IRAK1 ,p-IRAK1 and IRAK4 expression (protein) in THP-1 cells was significantly increased by stimulation of anti-β2GPI/β2GPI complex (100μg/ml) .IRAK1/4 inhibitor (50 μmol/L ) could decrease the levels of TF and p-IRAK1 in THP-1 cells induced by anti-β2GPI/β2GPI complex .Conclusion :IRAK1 and IRAK4 can be activated and contributed to TF expression of THP-1 cells induced with anti-β2GPI/β2GPI complex .关键词
抗磷脂综合征/anti-β2GPI/β2GPI/IRAK1/IRAK4/组织因子分类
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文海平,周红,许国莹,郭东琳,周芳,陈东东,解鸿翔..信号分子IRAK1/4在anti-β2GPI/β2GPI复合物诱导THP-1细胞表达TF中的作用探讨[J].中国免疫学杂志,2011,27(4):295-299,5.基金项目
本文受国家自然科学基金项目(No.30670907、30971301)和江苏大学学生科研课题项目(09A081)资助 （No.30670907、30971301）
