中国免疫学杂志2011，Vol.27Issue(4)：312-315,4.DOI:10.3969/j.issn.1000-484X.2011.04.006
微型双功能抗体在裸鼠体内介导人T细胞杀伤白血病细胞
Specific targeted cytotoxicity to resistant leukemia cells mediated by anti-CD3/anti-CD19 diabody
摘要
Abstract
Objective :To study the specific targeted cytotoxicity to resistant leukemia cells medidated by anti-CD3 /anti-CD19 diabody . Methods :Human peripheral blood lymphocytes (PBL ) were isolated using Ficoll-Hypaque density-gradient centrifugation from heparinized blood of healthy volunteers and monocytes were depleted by adherence to plastic flasks .Also releasing of perforin and granzyme A ,expressing the membrane molecular CD25 and CD69 on activated T cells of the same group ,and apotosis of these T cells were analyzed by qPCR or FACS respectively .The effect of the anti-CD3/anti-CD19 diabody mediated lysis of Raji cells was assayed by human leukemia nude mice xenograft model in vivo .Results :Diabody increased the cytotoxity of T cells in vitro apparently than the control groups ( P＜ 0 .05 ) .so the secretion of the perforin and Granzyme A ,CD25 and CD69 expressed on activated T cells were increased much more than in the other groups ( P＜0 .05 ) . Furthermore ,the Diabody showed improved tumor inhibiting activities ( P＜0 .05 ) and improved antitumor activity in nude mice bearing Raji xenografts .Conclusion :The diabody is proved to be a potent agent for mediating T lymphocyte cytotoxicity to leukemia cells in vitro and in vivo关键词
双功能抗体/CD19/CD3/非霍奇金淋巴瘤/免疫治疗分类
医药卫生引用本文复制引用
范冬梅,杨铭,赵英新,许元富,熊冬生,陈礼平,王敏..微型双功能抗体在裸鼠体内介导人T细胞杀伤白血病细胞[J].中国免疫学杂志,2011,27(4):312-315,4.基金项目
本文受国家自然基金项目资助(30971291)和天津市科技发展计划项目(05YFGZGX02800) （30971291）
