中国免疫学杂志2011，Vol.27Issue(4)：325-329,5.DOI:10.3969/j.issn.1000-484X.2011.04.009
红藤对LPS诱导流产小鼠的保胎作用及子宫巨噬细胞的影响
Effects of sargentodox cuneata on tocolysis role and the macrophages in the uteri of abortion mice induced by LPS
摘要
Abstract
Objective :To explore the immunosuppression effects and tocolysis of sargentodox on abortion mice induced by LPS .Methods :Mice of Kunming (55 mice) were divided into control group (group A ,10 mice) and expenmental group .Experimental group were divided into group B (intraperitoneal injection LPS ) ;group C (ingestion sargentodox ) ,and group D (ingestion sargentodox and intraperitoneal injection LPS ) ,each group of 15 mice .Then the pregnancy results were obversed ,and the varity of α-NAE+ ,CD14+ .CD204+ macrophages and TNF-α in the uteri were identified by enzyme-histochemistry ,immunohistochemistry and ELISA .Results :The abortion rate of group B is 100％ ( P＜ 0.01 ) ,The embryo resorbing rate is 100％ ( P＜0 .01 ) ,but there was the decreased abortion rate of 13 .30％ in group D .The embryo resorbing rate decreased to 12 .56％ .The number of α-NAE+ and CD14+ macrophages in the uteri of gestation mice of group B was greatly increased comparing with group A ( P＜0 .01 ) .These effects outside of myometrium of group D were remarkably increased comparing with group A ( P＜ 0 .01 ) .but there was no distinct difference in the inside of myometrium and function layer .The number of CD204+ macrophages in group C and D was greatly increased comparing with group A and B ( P＜0 .01 ) .The TNF-α contents in the uteri of mice in group B were greatly increased comparing with group A ( P＜0 .01) ,but close to nonnal levels in group D .Conclusion :The effect of miscamage induced by LPS can be antagonized by sargentodox ,through decreasing the quantity ,distribution and sub-groups of macrophages in the uteri of gravidity mice .Inhibited secretion of TNF- α is one of the mechanisms for maintaining normal embryonate development .关键词
红藤/巨噬细胞/CD14/CD204/TNF-α/流产/小鼠分类
医药卫生引用本文复制引用
王卫华,闫坤,王丽霞,吴雷振,张瑞平,张顺利..红藤对LPS诱导流产小鼠的保胎作用及子宫巨噬细胞的影响[J].中国免疫学杂志,2011,27(4):325-329,5.基金项目
本文受河南省科技攻关项目(No.0524420040)和河南基础与前沿技术项目(No.092300410193)资助 （No.0524420040）
