山东医药2011，Vol.51Issue(1)：1-3,3.
hTERT启动子调控下PE38KDEL表达载体的构建及其在MiaPaCa2人胰腺癌细胞中的表达
Construction and expression of the vector of PE38KDEL under the control of the hTERT promoter in MiaPaCa2 human pancreatic cancer cell lines
摘要
Abstract
Objective To construct an expressive vector of PE38KDEL under the control of the hTERT promoter and to investigate the expression of this gene in human pancreatic cancer cell lines. Methods hTERTp promoter was identified by enzyme digested and sequencing. Sub clone the synthetical PE38KDEL gene into MCS of plRES2-EGFP and hTERTp IRES2 -EGFP, verified by enzyme digested and RT-PCR. Results The plasmids were successfully constructed. Both Mia PaCa2 and WI-38 could express PE38KDEL after transfected with pPE38KDEL-IRES2-EGFP, but only the MiaPaCa2 cell expressed the PE38KDEL after tranfected with phTERTp-PE38KDEL-IRES2-EGFP plasmid. Conclusions The expressive vector of PE38KDEL under the control of hTERT promoter is successfully constructed and the expression of PE38KDEL gene can be targeted in the MiaPaCa2 human pancreatic cell line by the hTERTp. This can provide some experimental evi dences for the gene-targeted therapy of human pancreatic cancer.关键词
hTERT启动子/PE38KDEL/胰腺肿瘤/基因疗法分类
医药卫生引用本文复制引用
李宝玉,陈剑秋,孙晋津..hTERT启动子调控下PE38KDEL表达载体的构建及其在MiaPaCa2人胰腺癌细胞中的表达[J].山东医药,2011,51(1):1-3,3.基金项目
教育部博士点基金项目(20060062008) （20060062008）
天津市自然科学基金项目(07JCYBJC10300). （07JCYBJC10300）
