疑难病杂志2011，Vol.10Issue(2)：126-128,封3,4.DOI:10.3969/j.issn.1671-6450.2011.02.022
通络化纤颗粒对肺纤维化大鼠NF-κB、α-SMA含量的影响
Effect of Tongluohuaxiankeli on NF-κB and α-SMA in pulmonary fibrosis rats
杨质秀 1刘金艳 1韩迪 1杨明耀 2李秀荣 3许宏连 1李燕 1徐婧1
作者信息
- 1. 150001,黑龙江省中医研究院南岗分院呼吸科
- 2. 164000,黑龙江省北安市中医院呼吸科
- 3. 150036,哈尔滨,黑龙江省医院总院病理科
- 折叠
摘要
Abstract
Objective To investigate the effect of Tongluohuaxiankeli ( TLHXKL ) on NF-kB and α-SMA in rats with pulmonary fibrosis. Methods Sixty healthy Wistar rats were randomly divided into 5 groups, normal group, model group, positive control group and two TLHXKL groups which were treated with high and moderate dose of TLHXKL respectively. Except those rats in normal group, all rats were established pulmonary fibrosis by intra tracheal administration of bleomycin. Different medicines were administrated in different groups from the 1 st day after the models were established. Rats were sacrificed in batches at 2 time points of the 7th and 28th day for observing the pathological changes of lung under light microscope with HE staining and identifying NF-kB and α-SMA in lung tissue by immunohistochemical stain and image quantitative analysis. Results 7 th and 28 th day after model was established, positive expression of NF-kB and α-SMA was not found in normal group,but was found in model group. The NF-kB and α-SMA content in pulmonary tissue of the TLHXKL group and the positive control were remarkably decreased than those of model group in every stage( P ＜0.01 ). For the TLHXKL group, each dose group could decrease the content of NF-kB and α-SMA in lung tissue, however, the effect of high dose group were more effective. The effect of TLHXKL was superior to that of positive control in every stage( P ＜ 0.05 ). Pathological observation showed that inflammatory reaction was obvious in model group, and the fibrosis degree was severe, while the changes were significantly attenuated in positive control guoup and TLHXKL group. Conclusion TLHXKL can effectively reduce the NF-kB and α-SMA content in pulmonary fibrosis rats.关键词
通络化纤颗粒/肺纤维化/NF-κB/α-SMA/大鼠引用本文复制引用
杨质秀,刘金艳,韩迪,杨明耀,李秀荣,许宏连,李燕,徐婧..通络化纤颗粒对肺纤维化大鼠NF-κB、α-SMA含量的影响[J].疑难病杂志,2011,10(2):126-128,封3,4.
