中国人兽共患病学报2011，Vol.27Issue(1)：23-28,6.
牛副流感病毒3型的分离鉴定及感染牛抗体消长规律的研究
Isolation and identification of bovine parainfluenza virus type 3 and the growth and decline antibody titer of the infected dairy cattle
周玉龙 1吴海涛 1任亚超 2耿静 1王密 1谢金鑫 1朴范泽1
作者信息
- 1. 黑龙江八一农垦大学动物科技学院,大庆,163319
- 2. 哈尔滨医科大学大庆校区,大庆,163319
- 折叠
摘要
Abstract
To study this pothogeny and neutralization antibody titer change of infected cattle, the milk and blenna narium of fever symptomatic dairy cattle were collected in this study to isolate virus. The virus were identified by observing the CPE on MDBK cell, scanning electron microscope, physical and chemical properties test, hemagglutination test, viral neutralization test, and RT-PCR. The neutralization antibody of five pathogenetic cattle was monitored by means of viral neutralization test for thirteen month. A RNA virus isolated from the samples could lead CPE of cell fusion with envelope and projections from the virion envelope but no hemagglutination, and it could neutralize antiserum for the reference bovine parainfluenza virus type 3 (BPIV-3) BN-1 strain. The homology was up to 99.6％ between HN gene and Japanese BPIV-3 (GenBank accession number Y00114 and D84095). The results of neutralization antibody titer of cattle monitored showed that antibody titer for convalescent period was four times more than acute stage. Meanwhile the neutralized antibody titer of infected bovine could be maintained at 1∶25tol∶27 for 6 to 8 months, then began to descent, and finally remained stable at 1∶22 to 1∶23. Overall, the isolated strain was identified as BPV-3. It was further confirmed that the BPIV-3 was one of etiological factors for the hyperthermia of cattle herd. The neutralizing antibody titer could be maintained at a high level for at least six months.关键词
牛副流感病毒3型/分离鉴定/中和抗体分类
生物科学引用本文复制引用
周玉龙,吴海涛,任亚超,耿静,王密,谢金鑫,朴范泽..牛副流感病毒3型的分离鉴定及感染牛抗体消长规律的研究[J].中国人兽共患病学报,2011,27(1):23-28,6.
