中国比较医学杂志2011，Vol.21Issue(1)：45-47,53,后插2,5.DOI:10.3969/j.issn.1671.7856.2011.01.009
利用人脐带血干细胞制备人鼠肝组织嵌合体模型
Preparation for the Model of Mouse-Human Liver Tissue Chimeric by Human Cord Blood Stem Cells
摘要
Abstract
Objective To establish the model of human chimeric mouse infected by HBV through using human cord blood stem cell. Methods Human cord blood stem cells were twice injected through tail vein into the body of each nude mouse. Hepatic tissues were get at the 7th, 14th and 21th days to test the growth of human hepatic cells by AFP immunohistochemistry. In addition, mice were infected by HBV to quantitively test the serum AFP and ALB by RIA.Results The mouse hepatic AFP, existing in the cytoplasm, could express continuously. AFP positive cells could be observed at the 7th, 14th and 21st days. Meanwhile, the infected mouse hepatic tissues showed HBsAg positive by immunohistochemistry. It showed significant difference ( P ＜ 0. 05 ) between experimental and control groups. Conclusions Human cord blood stem cells can be transplanted into mouse liver and differentiate into human hepatic cells which are able to survive and to be infected by HBV in chimeric form.关键词
人鼠嵌合/人脐血干细胞/甲胎蛋白/白蛋白分类
医药卫生引用本文复制引用
周笛,范沛,张建,顾为望,姚刚,刘光泽..利用人脐带血干细胞制备人鼠肝组织嵌合体模型[J].中国比较医学杂志,2011,21(1):45-47,53,后插2,5.基金项目
广东科技项目(No.2007A060305004). （No.2007A060305004）
