中国临床药理学杂志2011，Vol.27Issue(1)：37-41,5.
LY294002联合姜黄素对人膀胱癌EJ细胞的体外抑制作用
Inhibitory and apoptosis effect of human bladder carcinoma EJ cells induced by curcumin combined with LY294002
王晶宇 1王志平 2赵俊丽 2张哲文1
作者信息
- 1. 兰州大学,基础医学院,病理生理学研究所,兰州,730000
- 2. 兰州大学第二医院,泌尿外科研究所,兰州,730030
- 折叠
摘要
Abstract
Objective To study the anti -proliferation and apoptosis induction effect of curcumin combined with LY294002 on human bladder cancer EJ cells.Methods The growth inhibition of curcumin and LY294002 alone or in combination on bladder cancer EJ cells by MTT assay.Effects of curcumin and LY294002 alone or in combination with phosphatidylinositol - 3 - kinases ( PI3 K) inhibitor LY294002 on apoptosis of EJ cells were detected by flow cytometry (FCM).Curcumin and LY294002 alone or in combination regulated caspase -3 and PARP protein expression in EJ cells by Western blotting analysis.Results The inhibition ratio of proliferation of EJ cells was increased significantly by the combination application of curcumin with LY294002 (P ＜ 0.05 ).The apoptotic ratio of EJ cells was increased by curcumin in combination with LY294002.Conclusion The tumor - killing effect of curcumin on bladder cancer EJ cells could be increased significantly by the combination application of inhibiting phosphatidylinositol - 3 - kinases/protein kinases (PI3K/Akt) signal transduction and improving apoptosis may be involved in its inhibitory effects.关键词
磷脂酰肌醇-3激酶/蛋白激酶B/LY294002/姜黄素/膀胱癌/细胞凋亡分类
医药卫生引用本文复制引用
王晶宇,王志平,赵俊丽,张哲文..LY294002联合姜黄素对人膀胱癌EJ细胞的体外抑制作用[J].中国临床药理学杂志,2011,27(1):37-41,5.
