四川大学学报（医学版）2011，Vol.42Issue(1)：52-55,4.
葛根素对大鼠局灶性脑缺血后海马CA1区NMDA受体的影响及其意义
Effect of Puerarin on the Expression of NMDA Receptor in the Hippocampus CA1 Region after Focal Cerebral Ischemia in Rats
摘要
Abstract
Obiective To study the antagonistic action of Puerarin against the excitatory amino acid toxicity,and to further explore its brain protection mechanisms.Methods Focal cerebral ischemia model was set up with middle cerebral artery occlusion by intraluminal block in this study.After cerebral ischemia, Puerarin was administered at different time point.The volume of cerebral ischemia was assessed by TTC stain.NR1 positive neurons in hippocampus CAl region was determined by immunohistochemistry SABC method.Results The cerebral ischemia volumes were smaller in 2 h and 12 h model treatment groups(P＜ 0.05)than those in model control groups, but no significant differences were observed in 24 h model treatment group.Compared with sham-operation group, the NRl-positive cells in himppocampal CA1 region were increased obviously(P＜0.05) in both model control and model treatment groups.Compared with model control groups, the NR1-positive cells in himppocampal CA1 were decreased obviously (P＜0.05) in 2 h and 12 h treatment groups.Conclusion The treatment of Puerarin within 12 h after ischemia can cut down the expression of NMDA receptor.This indicates that the puerarin treatment in earlier period after ischemia can indirectly rivalry toxic effect of excitatory amino acids, relieve neural injury.关键词
葛根素/大鼠/脑缺血/兴奋性氨基酸/N-甲基-D-天冬氨酸受体Key words
Puerarin / Rat / Cerebral ischemia / Excitatory amino acids / NMDA receptor引用本文复制引用
张茹,郭荷娜,吴海琴,成红学,王虎清..葛根素对大鼠局灶性脑缺血后海马CA1区NMDA受体的影响及其意义[J].四川大学学报（医学版）,2011,42(1):52-55,4.基金项目
陕西省中医现代化基金(编号:2006K16-G5(3)]资助 （编号:2006K16-G5(3）
