中医学报2011，Vol.26Issue(1)：57-59,3.
灯盏乙素对过氧化氢致血管内皮细胞损伤的作用及其机制研究
The Study of the Protect Effect of Scutellarin on the Endothelial Cells Induced by Hydrogen Peroxide
摘要
Abstract
Objective: To explore the protective effects of scutellarin on vascular endothelial cell injury induced by oxidative hydrogen.Methods: Preincubate with different concentrate of scutellarin and VE, 1 mmol · L-1 Peroxide hydrogen ( H2O2 ) was used to induce injury of human umbilical vein endothelial cells (HUVEC). The cell survival rate was evaluated with MTT assay to explore the protectire effect of scutellarin on injury of vascular endothelial cells. The activity of superoxide dismutasc (SOD) , glutathione peroxidase ( GSH - PX) and catalase (CAT) was detected in H2O2 - induced HUVEC injury. Results: HUVEC was incubated with I mmol · L-1 H2O2 for 4 h, the cell viability decreased significantly (P ＜ O. 05 vs normal control group); scutellarin can promote the cell viability of HUVEC induced by H2O2(P ＜ O. 05 vs H2O2 -injury group); scutellarin could concentration -dcpendently protect the activity of SOD and GSH -PX (P ＜ 0.05 vs H2O2 injury group). Conclusion: Scutellarin showed protective effects on vascular endothelial cells induced by oxidative stress and the probable mechanisms may be closely associated with its promoting the activity of antioxidases.关键词
过氧化氢/灯盏乙素/内皮细胞/MTT法/超氧化物歧化酶/GSH-PX/过氧化氢酶Key words
H2O2/ scutellarin/ Endothelial cell/ MTT method/ SOD/ GSH - PX/ CAT分类
医药卫生引用本文复制引用
崔琳,余海滨,李强,刘卫红,马春芬..灯盏乙素对过氧化氢致血管内皮细胞损伤的作用及其机制研究[J].中医学报,2011,26(1):57-59,3.基金项目
云南省自然科学基金项目(编号:2004C0019Q) （编号:2004C0019Q）
