解放军医学杂志2011，Vol.36Issue(1)：31-34,4.
低pH液复灌对兔缺血再灌注心肌内质网应激与细胞凋亡的影响研究
Effects of acidic reperfusion fluid on myocardial endoplasmic reticulum stress and apoptosis after ischemia/reperfusion in rabbits
摘要
Abstract
Objective To determine the effect of acidic reperfusion fluid in cardiopulmonary bypass (CPB) on the myocardial endoplasmic reticulum stress (ERS) and apoptosis after ischemia/reperfusion in rabbits. Methods Twenty-four adult New Zealand white rabbits of both sexes weighing 2.5～3.0 kg were randomly divided into 4 groups (6 each). Control group (C); CPB for 3 hours without aortic clamping; pHT. 4 group and pH6. 9 group: hearts were subjected to ischemia for 40min followed by 2 hours of reperfusion, and then perfused with Krebs-Henseleit bicarbonate buffer solution, respectively, at pHT. 4 and pH6o 9 in the first 2min of reperfnsion; ischemic postconditioning group (I-postC) : 40min after aortic clamping, 6 cycles of 10-second reperfusion/10-second occlusion were given in the first 2min of 2-hour reperfusion. Cardiac troponin I (cTnI) concentration was determined with ELISA, apoptosis of myocardial cell was detected by TUNEL, the expressions of glucose-regulated protein 78(GRP78)and calreticulin (CRT) mRNA were detected by RT-PCR,and the expressions of caspase-12 and C/EBP homologous protein (CHOP) were assayed by immunohistochemistry. Results Compared with the C group, the cTnI concentration, apoptotic rate, expressions of GRP78, CRT mRNA, caspase-12 and CHOP of the other groups increased significantly (P＜0. 05). Compared with the pHT. 4 group, all the indexes were significantly lower in pH6. 9 group and I-postC group (P＜0. 05), but no significant difference was found between the latter two groups (P＞0. 05). Conclusion Acidic reperfusion may protect the heart against I/R injury, regulate ERS response, inhibit excessive ERS, and alleviate ERS-mediated apoptosis during I/R.关键词
氢离子浓度/再灌注损伤/内质网应激/细胞凋亡Key words
hydrogen-ion concentration/ reperfusion injury/ endoplasmic reticulum stress/ apoptosis分类
医药卫生引用本文复制引用
杨海燕,刘新伟,马亚飞,王群英,刘柳..低pH液复灌对兔缺血再灌注心肌内质网应激与细胞凋亡的影响研究[J].解放军医学杂志,2011,36(1):31-34,4.基金项目
重庆市卫生局自然科学基金(2009-2-344) （2009-2-344）
