南京医科大学学报（自然科学版）2011，Vol.31Issue(1)：21-25,5.
HHV-8 K13基因编码蛋白在人血管内皮细胞和前列腺癌细胞系中的表达及其对细胞增殖影响的初探
The eukaryotic expression of HHV-8 K13 and its effect on the proliferation of human endothelial and prostate cancer cells
摘要
Abstract
Objective:To investigate the expression protein of HHV-8 KI3 in human endothelial and prostate cancer cells respectively, and evaluate the effect on the proliferation of the two cell lines. Methods:The recombinant plasmid PEF-KI3-Flag-IRES/Puro was transianfiy trancfected into EA.HY926 cells and PC-3 cells. The expression of KI3 protein in both cells was analyzed by Western blot. The function of K13 protein in cell cycle and cell proliferation were measured by flow cytometry and MTI assay, respectively. Results:The specific band of K13 protein was detected at the expected place by Western blot. In addition, the results of the flow cytometry and MTT assay showed that the HHV-8 K13 protein could significanfiy inhibit the proliferation in human endothelial and prostate cancer cells. Conclusion:HHV-8 K13 protein might inhibit the growth of human endothelial and prostate cancer cells.关键词
前列腺癌/人类疱疹病毒8型/K13/PC-3/内皮细胞Key words
prostate cancer/ HHV-8/ K13/ PC-3/ endothelial cell分类
生物科学引用本文复制引用
糜远源,华立新,冯宁翰,闵治超,周峰,朱小飞,王子盾,程伟,卢春..HHV-8 K13基因编码蛋白在人血管内皮细胞和前列腺癌细胞系中的表达及其对细胞增殖影响的初探[J].南京医科大学学报（自然科学版）,2011,31(1):21-25,5.基金项目
中国博士后基金资助(20080441064) （20080441064）
