南京医科大学学报（自然科学版）2011，Vol.31Issue(1)：8-12,20,6.
骨髓细胞不同组份形成成纤维细胞集落形成单位的效率及1,25(OH)2D3和PGE2在其中的调节作用
Efficiency of colony forming unit-fibroblastic formed by the different fraction of bone marrow cells and regulation of 1,25(OH)2D3 and PGE2 in colony formation
摘要
Abstract
Objective: To determine whether bone marrow mesenchymal stem cells (BM-MSCs) are presented in the different fractions of bone marrow cells and whether the efficiency of colony forming unit-fibroblastic (CFU-f) formed by BM-MSCs is regulated by 1,25(OH)2D3 and prostaglandin E2(PGE2). Methods:Total bone marrow cells or non-adherent bone marrow cells derived from total bone marrow cell cultures for 1 day were separated into the mononuclear cell fraction and the granulocyte/erythrocyte fraction or the mononuclear cell fraction, the granulocyte fraction and the erythrecyte fraction by density-gradient centrifugation and were cultured in the absence or presence of 10-8mol/L 1,25 (OH)2D3 or 10-7 mol/L PGE2 for 10 days. The resulting cells were stained with methylene blue and the number of CFU-f was counted. Results: ①The CFU-f formed by mononuclear cell fraction accounted for about 10％ of the total CFU-f, and CFU-f formed by granulocyte/erythrocyte fraction, granulocyte fraction and erythrocyte fraction accounted for about 90％ ,47％ and 35％ of the total CFU-f, respectively; ②The fractions derived from the non-adherent bone marrow cells accounted for about 71％ of the total CFU-f, which was more than those formed by the fractions derived from total bone marrow cells;③ Treatment of 1 ,25(OH)2D3 and PGE2 enhanced the CFU-f formation of the mononuclear fraction and the granulocyte fraction derived from total bone marrow cells and the mononuclear cell fraction, the granulocyte fraction and the erythrocyte fraction derived from non-adherent bone marrow cells. Conclusion: Our results indicate that BM-MSCs are presented in the different fractions of bone marrow cells,and 1 ,25(OH)2D3 and PGE2 play a rele in stimulating proliferation of BM-MSCs.关键词
骨髓细胞组份/间充质干细胞/1,25(OH)2D3/PGE2Key words
bone marrow cell fractions/ mesenchymal stem cells/ 1,25(OH)2D3/PGE2分类
生物科学引用本文复制引用
王嵘,苗登顺,季吉..骨髓细胞不同组份形成成纤维细胞集落形成单位的效率及1,25(OH)2D3和PGE2在其中的调节作用[J].南京医科大学学报（自然科学版）,2011,31(1):8-12,20,6.基金项目
教育部高等学校博士学科点专项科研基金(20070312010) （20070312010）
