南京医科大学学报（自然科学版）2011，Vol.31Issue(1)：58-60,75,4.
与胶原纤维的作用是M(U)ller细胞黏附于玻璃体的主要机制
Interaction with collagen fiber is the main mechanism of M(U)ller cell adhesion to vitreous gel
摘要
Abstract
Objective: To investigate the mechanism of M(U)ller cell adhesion to vitreous gel. Methods: Porcine M(U)ller cells were extracted and cultured, identified with immuno-recognition with glial fibrillary acidic protein (GFAP),vimentin and glutamine synthetase (CS) antibody. Cells were collected and incubated with/without integrinα2βl and CD44 antibody before application to fresh porcine vitreous for 2 hours, and then washed with PBS for 3 times. Remaining cells on the vitreous were counted. Results: ① Cells collected were 97.7％ positive for GFAP,94.6％ positive for vimentin,86.0％ positive for GS, respectively. Cells adhered to vitreous after 2 hours of culture, formed net-like structure and enter vitreous gel after 72 hours; ② Pre-incubation with integrinα2βl antibody significantly decreased the remaining cell, while no such significance was observed in the cells incubated with CD44 antibody. Conclusion: M(U)ller cell can adhere to vitreous and proliferate, in which the interaction with type Ⅱ collagen is an important mechanism.关键词
M(U)ller细胞/玻璃体/整联蛋白α2β1/CD44Key words
M(U)ller cell/ vitreous/ integinα2βl /CD44/分类
生物科学引用本文复制引用
陆融,姚勇,乔伟振,赵伟..与胶原纤维的作用是M(U)ller细胞黏附于玻璃体的主要机制[J].南京医科大学学报（自然科学版）,2011,31(1):58-60,75,4.基金项目
南京医科大学科技发展基金重点项目(09NJMUZ52) （09NJMUZ52）
