南京医科大学学报（自然科学版）2011，Vol.31Issue(1)：26-30,5.
DNA甲基化异常对mir-615-5p在胰腺癌细胞株PANC-1中表达影响
Abnormal DNA methylation in pancreatic cancer cell line PANC-1 affects mir-615-5p expression
摘要
Abstract
Objective: To observe the abnormal DNA methylation inhibits the miRNA expression in pancreatic cancer cell line,and DNA methyltransferase inhibitor on the miR-615-5p of the methylation status and expression in the pancreatic cancer cell line PANC-1. Methods: Methylation microarray was used to screen in pancreatic cancer cell line PANC-1 in the abnormal methylation of miRNA. PANC-1 cells were treated with demethylating agent 5-aza-2-adeoxycytidine (5-aza-dC) in vitro, Methylation specific polymerase chain reaction (methyla-tion- specific PCR, MSP) combination of bisulfite modified DNA sequencing method (bisulfite-sequencing PCR, BSP) were used to detect miR-615-5p methylation changes before and after treatment. The changes of miR-615-5p expression was detected by quantitative RT-PCR before and after treatment. Results: Methylation was significanfiy higher in pancreatic cancer cell line PANC-1 than in normal tissue by MSP and BSP methods. miR-615-5p reduction in methylation was associated with the expression reactivation in the pancreatic cancer cells PANC-1 treated with demethylation agent 5-asa-dC. Condlusion: This study demonstrates that aberrant DNA methylation of the miR-615-5p CpG island is closely linked to their inappropriate silencing in pancreatic cancer cell line PANC-1.关键词
DNA甲基化/miR-615-5p胰腺癌/5-氮杂胞苷Key words
DNA methylation/ miR-615-5p/ pancreatic cancer/ 5-aza-dC分类
生物科学引用本文复制引用
朱墨,高文涛,钱祝银,苗毅..DNA甲基化异常对mir-615-5p在胰腺癌细胞株PANC-1中表达影响[J].南京医科大学学报（自然科学版）,2011,31(1):26-30,5.基金项目
江苏省自然科学基金(BK2006241) （BK2006241）
国家自然科学基金(30500492) （30500492）
