解放军医学杂志2011，Vol.36Issue(1)：45-49,5.
复方奥硝唑甲磺酸培氟沙星牙周缓释制剂对雌性大鼠早期胚胎发育毒性的影响
Studies on the toxic effects of periodontal sustained release drug containing ornidazole and pefloxacin mesylate on early embryonic development of SD rat
摘要
Abstract
Objective To evaluate the toxic effects of periodontal sustained release drug containing omidazole and pefloxacin mesylate on early embryonic development of SD rats. Methods A total of 100 female SD rats were randomly divided into negative control,low-, medium-, high-dose group and intervention group (20 each). Rats in low-, medium- and high-dose group were fed daily with the sustained release drug at 1, 4, and 8 g/kg respectively; those in negative control group were fed daily with distilled water from the 14th day before mating to the 7th day of pregnancy continuously, and those in intervention group received cyclophosphamide (40mg/kg) by intraperitoneal injection for 5 successive days. During this period, the general status, mating, pregnancy, coefficient of ovary and uterus,the numbers of corpus luteum, nidation, live births, stillbirths, absorbed embryo, prenidatory and postnidatory mortality, serum testosterone (T) and estradiol (E2)were determined respectively. Histopathologic examination of the ovary and uterus,immunohistochemical observation of ovaries for proliferating cell nuclear antigen (PCNA) and Bcl-2 associated X protein (Bax) were also performed respectively. Results The general status of those rats was good except one in the low-dose group and one in the intervention group died on the 14th day of administration, and one in negative control and one in high dose group died on the 5th day of pregnancy,respectively. The body weight of animals decreased significantly (P＜0.05) from the 5th day of pregnancy in high dose group compared with negative control group. Compared with those parameters mentioned above, except the coefficient of ovary, which was lower in the 4 treatment groups than in negative control group, no significant difference was found between the negative control group and intervention and drug treatment groups (P＞0. 05). The serum T level in medium-and high-dose group and the E2 level in high-dose group declined compared to that in negative control group (P＜0. 05). Conclusions Although the periodontal sustained release drug containing ornidazole and pefloxacin mesylate shows no toxicity to the early embryonic development of SD rats, the high dose drug has certain toxicity to ovary.Declined serum concentrations of T and E2, reduced expression of PCNA, and increased Bax may be the causes of the toxicity.关键词
牙周组织/迟效制剂/生殖毒性/胚胎发育Key words
periodontium/ delayed-action preparations/ reproductive toxicity/ embryonic development分类
医药卫生引用本文复制引用
董正谋,刘鲁川,刘宁,刘锐,张海元,孔耀,敖琳..复方奥硝唑甲磺酸培氟沙星牙周缓释制剂对雌性大鼠早期胚胎发育毒性的影响[J].解放军医学杂志,2011,36(1):45-49,5.基金项目
重庆市科技攻关计划项目(CSTC2009AC5019) （CSTC2009AC5019）
