摘要
Abstract
Objective To investigate the interventional effects of pretreatment with total polyphenols extracted from Toona sinensis Roem on the injury induced by myocardial ischemia-reperfusion in rats. Metlods Fifty SD rats were randomly divided into 5 groups: sham operation group, model group and 3 pretreatment groups in which the rats were treated with total polyphenols in 3 different doses. Rats in sham operation and model group were treated with 5 sodium carboxy methyl cellulose, and in 3 pretreatment groups the rats were treated with 50, 100 and 200mg/kg of total polyphenols, respactively, by gavage for 7 days, and then the myocardial ischernia/reperfusion injury was induced by ligating the left coronary artery (LAD) for 30min followed by a 120 min reperfusion in anesthetized rats. Rats in sham operation group were operated with placing the ligature but without ligating. ECGs were recorded before and 30 min after ligating LAD,and 30, 60 and 90min after reperfusion. The levels of J point at different time were observed and compared. After reperfusion, the blood was collected from abdominal aorta to prepare serum samples. The activity of CK, the level of cTnI, TXB2 and 6-keto-PGF1a in serum were then determincd. Finally, the hearts of rats below the ligature were cut into 5 slices and stained with 1％ TTC for 15 min, the extent of myocardial infarction was calculated. Results Compared with that in sham operation group, the J point of ECG elevated obviously in model group, the activity of serum CK and the contents of cTnI and TXB2 increased markedly, while the 6-keto-PGF1a level declined significantly (P＜0.05), and notable myocardial infarction was apparent. Compared with those in model group, the height of J point descended, the activity of CK and the level of cTnI and TXB2 in serum decreased, while the level of 6-keto-PGF1a increased, and the degree of myocardial infarction was alleviated in the 3 pretreatment groups. All the data were found to have statistically significant differences.Conclusion Total polyphenols extracted from Toona sinensis Roem may play an interventional effect on the injury induced by myocardial ischemia/reperfusion of rats.关键词
香椿/总多酚/心肌缺血/心肌再灌注损伤Key words
Toona sinensis / total polyphenols/ myocardial ischemia/ myocardial reperfusion injury分类
医药卫生
