中医药信息2011，Vol.28Issue(1)：24-26,3.
杜仲抗UVB致HaCaT细胞光老化活性部位的初步筛选
Screening on Activity Part of Eucommia for Resistance Photoaging from UVB Induced Damage
摘要
Abstract
Objective :To ensure the effective part of Eucommia for anti - photoaging. Methods: Ethanol extract of Eucommia macroporous resin prepared by different parts of the concentration gradients of ethanol. The cell model of photoaging was acted with elution positions. The effective portion of anti - photoaging of eucommia was ensured by the index of cell morphology, cytoactive and LDH, MDA, SOD activity. Results: At the dosage of 125μg·mL-1 ,cytoactive were raised(P ＜0. 05 ) ;LDH activity were decreased(P ＜0. 01 ); MDA activity were decreased (P ＜ 0. 05 ) ;SOD activity wasincreased( P ＜ 0. 05 ) when 50％ elution site were added. Conclusion: Oxygen free radicals were scavenged; SOD activity were increased and MDA activity were decreased, and it protected membranes from damage and reduce the leakage of LDH. It proved that the eucommia site of 50％ ethanol eluting could resist cell photo- damage.关键词
杜仲/光老化/HaCaT细胞/有效部位分类
医药卫生引用本文复制引用
李建民,徐艳明,陈巧云,朱魁元,张宁..杜仲抗UVB致HaCaT细胞光老化活性部位的初步筛选[J].中医药信息,2011,28(1):24-26,3.基金项目
黑龙江省教育厅科学技术研究面上项目(11541332) （11541332）
黑龙江省中医药管理局课题(ZHY08-z33) （ZHY08-z33）
黑龙江中医药大学校科研基金(200919) （200919）
