四川大学学报（医学版）2011，Vol.42Issue(1)：10-14,5.
过氧化物酶体增殖物激活受体γ2在脂肪干细胞脂向分化过程中的表达
Expression Profiling of Peroxisome Proliferation Activate Receptor γ2 and Phosphorylation During Adipogenic Differentiation of Adipose-derived Stem Cells
摘要
Abstract
Objective To investigate PPARγ2 expression and phosphorylation involved in adipogenic differentiations of Adipose-derived Stem Cells (ASCs) with an aim to reveal the possible mechanisms of adipose development. Methods ASCs were obtained from the inguinal fat pads of GFP (green fluorescent protein) mice.The primary cells were cultured in histiocytic attachment cultivation. Adipogenic differentiation of the third generation of ASCs was induced with the conditional medium (α-MEM medium supplemented with 100 mL/L fetal bovine serum, 10-6 mol/L dexamethasone, 10-5 mol/L insulin, 0.2 mmol/L indomethacin, and 0.5 mmol/L 3 -isobutyl-l-methylxanthine). The levels of PPARγ2 mRNA and the PPARγ2 expression and phosphorylation at day 0, 1, 3, 5, 7, and 9 of induction were detected using RT-PCR, fluorescence-immunocytochemistry and western blot, respectively. Results The expression of PPARγ2 and phosphorylation were significantly up-regulated during adipogenic differentiation of ASCs. Conclusion PPARγ2 is the key regulator of adipose development. The regulation of PPARγ2 phosphorylation may promote adipogenic differentiation of ASCs.关键词
脂肪干细胞/过氧化物酶体增殖物激活受体γ2/磷酸化/脂肪分化Key words
Adipose-derived stem cells/ PPARγ2/ Phosphorylation/ Adipogenic differentiation引用本文复制引用
孙钦策,肖金刚,杨晓娟,田卫东..过氧化物酶体增殖物激活受体γ2在脂肪干细胞脂向分化过程中的表达[J].四川大学学报（医学版）,2011,42(1):10-14,5.基金项目
教育部博士点基金(20070610064)和国家自然科学基金(批准号30973348)资助 （20070610064）
