四川大学学报（医学版）2011，Vol.42Issue(1)：15-18,36,5.
基因芯片筛选结肠癌伊立替康耐药相关基因
Analysis of Gene Expression Patterns in an Irinotecan-resistance Colon Cancer Cell by cDNA Microarray
摘要
Abstract
Objective To study the mechanism of irinotecan-resistant colon cancer by analyzing the differential gene expression patterns with cDNA microarray. Methods Total RNA was purified from irinotecan sensitive colonic cancer cell line, SW480 and its irinotecan-resistance cell line, SW480/CPT. The cRNA retro transcribed from RNA were labeled with Cy3 fluorescence as probes. The probes were hybridized with Agilent gene chips and the fluorescence images of the chips were obtained with Axon 4000B scanner as well as analyzed with Genepix 3.0 software. The microarray results were confirmed by reverse transcription-polymerase chain reaction. Results Of the 1598 genes with altered expressions, there were 911 up-regulated genes and 687 down-regulated genes. Glutathione S-Transferase (GST) isoenzyme family GSTA such as GSTA1, GSTA2, GSTA3 and GSTA5 were significantly up-regulated. The expression levels of many Zinc finger protein family members (ZNF) were also differentially regulated. Conclusion GSTA and ZNF subunit genes might play an important regulation role in the irinotecan resistance of colon cancer.关键词
基因芯片/伊立替康/耐药/结肠癌/基因表达引用本文复制引用
陈曦,陈向征,刘明,郎楠,唐秋琳,陈济,赵晓斐,毕锋..基因芯片筛选结肠癌伊立替康耐药相关基因[J].四川大学学报（医学版）,2011,42(1):15-18,36,5.基金项目
国家自然科学基金(批准号30901719,30971519)资助 （批准号30901719,30971519）
