肿瘤防治研究2011，Vol.38Issue(1)：21-24,4.DOI:10.3971/j.issn.1000-8578.2011.01.006
肝癌细胞系中Oct4与Wnt/β-catenin和TGF-β信号通路的相互影响
Modulation of Oct4 to Wnt/β-catenin and TGF-β Signal Ways in HCC Cell Line
摘要
Abstract
Objective To investigate the relationships among Oct4 and Wnt/β-catenin as well as TGF-β signal ways in hepatocellular carcinoma(HCC) cell line HepG2. Methods RT-PCR was used to detect the expression of Oct4 and the genes in both Wnt/β-catenin and TGF-β signal ways in HCC cell lines and HCC specimens. RNAi was used to mediated knock-down the expression of Oct4 and TCF3 in HepG2, and the change of Wnt/β-catenin and TGF-β related genes were also detected by RT-PCR.Results Oct4 and the genes in both Wnt/β-catenin and TGF-β signal ways including β-catenin,Wnt10b, TCF3 and ELF, Smad3, Smad4 were expressed in HCC cell lines as well as in the specimens from HCC patients. After treating the Oct4 in HepG2 with siRNA, the expression of TGF-β family genes ELF, Smad3, Smad4 and Wnt/β-catenin family genes wnt10b,β-catenin reduced, while TCF3 increased. In reverse, knockingdown TCF3 led to an increase in the expression of Oct4 and TGF-β family genes. Conclusion The result of silencing Oct4 and TCF3 in HepG2 by siRNA suggested that there is a certain correlation among Oct4, Wnt/β-catenin and TGF-β family genes in HCC cell line HepG2, which reveals the role of interactions between Oct4 in HCC is more worthy to be researched.关键词
Oct4/Wnt/β-catenin/TGF-β/RNAi/肝细胞性肝癌分类
医药卫生引用本文复制引用
袁方均,周文波,邹灿,胡洪生,张志云,戴宗晴,张有顺..肝癌细胞系中Oct4与Wnt/β-catenin和TGF-β信号通路的相互影响[J].肿瘤防治研究,2011,38(1):21-24,4.基金项目
湖北省教育厅重点资助项目(D200724003),十堰市科技局科技攻关项目(十科发[2008]024D),郧阳医学院创新团队资助项目(2008CXG03) （D200724003）
