肿瘤防治研究2011，Vol.38Issue(1)：17-20,4.DOI:10.3971/j.issn.1000-8578.2011.01.005
人参皂苷CK对胃癌细胞株SGC-7901及其内源性VEGF的影响
Effects of Ginsenoside CK on Gastric Cancer SGC-7901 Cell Line and Endogenous VEGF Secreted by Tumor Cells
邓晶 1蒋永新 2寸英丽 3陈晓群 2万成亮3
作者信息
- 1. 650018,昆明,昆明医学院第三附属医院云南省肿瘤医院综合内科
- 2. 云南省肿瘤研究所
- 3. 昆明医学院第三附属医院,云南省肿瘤医院腹部外科
- 折叠
摘要
Abstract
Objective To investigate the effects of Ginsenoside CK on the cell proliferation、 cell cycle and endogenous VEGF of gastric cancer SGC-7901 cell line.Methods SGC-7901 cell line in cultural medium in vitro were given 50,25,12.5,6.25,3.125,1.5625 μg/ml Ginsenosde CK.The inhibitory rate of cell line was measured by MTT assay; cell apoptotic rate and cell phase were detected by flow Cytometry (FCM); the VEGF contents were detected by ELISA assay.Results It was found that Ginsenoside CK had significant inhibitory effect on the proliferation of SGC-7901 cell line, and this effect was dose-and-time-dependent; FCM assay indicated that apoptosis peak appeared evidently after the cells being interfered with Ginsenoside CK and most of cells were arrested in G0/G1 phase; The levels of VEGF in Ginsenoside CK groups were lower than that in the control group (P＜0.05).The level of VEGF in the groups of high concentration Ginsenoside CK were lower than that in the group of low concentration(P＜0.05), and the VEGF contents decrease with action time prolonging.Conclusion Ginsenoside CK dramatically inhibits proliferation of SGC-7901 cell line by inducing cell apoptosis and secretion of endogenous VEGF.Ginsenoside CK may become a potential drug in the prevention and treatment of gastric cancer.关键词
人参皂苷CK/胃癌细胞株SGIC-7901/凋亡/血管内皮生长因子分类
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邓晶,蒋永新,寸英丽,陈晓群,万成亮..人参皂苷CK对胃癌细胞株SGC-7901及其内源性VEGF的影响[J].肿瘤防治研究,2011,38(1):17-20,4.
