肿瘤防治研究2011，Vol.38Issue(1)：28-30,3.DOI:10.3971/j.issn.1000-8578.2011.01.008
人结肠癌裸鼠肝转移模型的建立
Establishment and Comparison of Colon Cancer Liver Metastasis Models in Nude Mice
摘要
Abstract
Objective To develop a reliable colon cancer liver metastasis model in nude mice. Methods Thirty nude mice were divided into two groups randomly, which were the liver injection group and spleen injection group, respectively containing 15 nude mice. Colon cancer liver metastasis models were made by liver injection and spleen injection, respectively. The average survival time was recorded. The metastasis of nude mice was detected pathologically. Results Both of the incidence rates of liver metastasis in the two groups were 100％. Survival time of the two groups showed no statistics significance. Anatomical study showed that there appeared a single giant tumor nodule appeared at every injection site in the liver injection group while multiple metastasis appeared nodules in livers in the spleen injection group. The pathological results indicated that the tumor cells pattern of liver metastasis was similar in the two groups and was in accordance with poorly differentiated colon adenocarcinorna. Conclusion Intrasplenic injection of colon cancer cells, which closely mimic the natural metastatic process, is a reliable method for producing colon cancer liver metastasis in nude mice and should be proved valuable in experimental study.关键词
结肠癌/肝转移/模型/裸鼠分类
医药卫生引用本文复制引用
袁岱岳,郭忠英,赵任,黄宝玉,梅广林,朱建伟..人结肠癌裸鼠肝转移模型的建立[J].肿瘤防治研究,2011,38(1):28-30,3.基金项目
国家自然科学基金资助项目(30771126) （30771126）
