西南国防医药2011，Vol.21Issue(1)：4-7,4.DOI:10.3969/j.issn.1004-0188.2011.01.002
重组人β-防御素3对铜绿假单胞菌生物膜结构影响的初步研究
Inhibitory effects of recombinant human beta-defensin 3 on biofilm structure of Pseudomonas aeruginosa:a preliminary study in vitro
摘要
Abstract
Objective To investigate the inhibitory effects of recombinant human heta - defensin 3( rhBD - 3 )on the hiofilm formation of Pseudomonas aeruginosa( PA ) in vitro. Methods Standard gelose radial diffusion assay was used to measure the minimal inhibitory concentration( MIC ) of rhBD - 3 on PA. Then the early and mature biofilms of PA inoculated on polycarhonate membrane were obtained using plate culture method. These BFs were randomly divided into four groups: blank control group and three groups treated with different concentration of rhBD - 3(1/2MIC ,2MIC and 4MIC , respectively ). The appearance of biofilms on vehicle surface was observed under scanning electron microscope( SEM ). Viable count was conducted by serial dilution method. Results The MIC of rhBD - 3 on Pseudomonas aeruginosa was 64 μg/ml. The amount of viable bacterial adhered to the vehicle surface decreased 8.16 and 24 h after rhBD - 3 treatment. Furthermore ,it decreased progressively with the increase of rhBD - 3 concentration. Small amounts of sporadic and free bacteria as well as patchy polyose compounds were seen in rhBD - 3 groups under SEM. However,large amounts of evenly distributed microcolony and free bacteria as well as the crosslinking of polyose compounds were found in hlank control group. Conclusion rhBD - 3can prevent PA from forming BF in vitro. It can also destroy the structure of this biofilm in early or mature stage.关键词
铜绿假单胞菌/生物膜/人β-防御素3/琼脂糖弥散抗菌法分类
医药卫生引用本文复制引用
周菁,陈章,田坤,吴奎,肖贞良..重组人β-防御素3对铜绿假单胞菌生物膜结构影响的初步研究[J].西南国防医药,2011,21(1):4-7,4.基金项目
全军"十一五"课题面上项目(06MB191) （06MB191）
成都军区"十一五"课题面上项目(MB07015) （MB07015）
