首页|期刊导航|中华医学杂志（英文版）|Keratinocyte growth factor phage model peptides can promote epidermal cell proliferation without tumorigenic effect
中华医学杂志（英文版）2010，Vol.123Issue(9)：1195-1200,6.DOI:10.3760/cma.j.issn.0366-6999.2010.09.017
Keratinocyte growth factor phage model peptides can promote epidermal cell proliferation without tumorigenic effect
Keratinocyte growth factor phage model peptides can promote epidermal cell proliferation without tumorigenic effect
摘要
Abstract
Background Keratinocyte growth factor (KGF) significantly influences epithelial wound healing. The aim of this study was to isolate KGF phage model peptides from a phage display 7-mer peptide library to evaluate their effect on promoting epidermal cell proliferation. Methods A phage display 7-mer peptide library was screened using monoclonal anti-human KGF antibody as the target. Enzyme linked immunosorbent assay (ELISA) was performed to select monoclonal phages with good binding activity. DNA sequencing was done to find the similarities of model peptides. Three-(4,5-dimethylthiazol-2-yl) -2,5-diphenyl tetrazolium bromide (MTT) assay, immunofluorescence assay and quantitative real-time PCR analysis were employed to evaluate the effect of the phage model peptides on epidermal cells. Results Thirty-three out of fifty-eight (56.9%) of the isolated monoclonal phages exhibited high binding activity by ELISA. Ten of fifteen obtained phage model peptides were similar to KGF or epidermal growth factor (EGF). MTT assay data showed that four (No. 1-4) of the ten phage model peptides could promote epidermal cell proliferation. The expression of keratinocyte growth factor receptor (KGFR) mRNA in the KGF control group and the two phage model peptide groups (No. 1 and No. 2) increased. Expression of c-Fos mRNA and c-Jun mRNA in the KGF control group increased, but did not increase in the four phage model peptide groups (No.1-4). Conclusion Four phage model peptides isolated from the phage display 7-mer peptide library can safely promote epidermal cell proliferation without tumorigenic effect.关键词
phage display peptide library/keratinocyte growth factor/peptide/cellular proliferation/wound healingKey words
phage display peptide library/keratinocyte growth factor/peptide/cellular proliferation/wound healing引用本文复制引用
ZONG Xian-lei,JIANG Du-yin,WANG Ji-chang,LIU Jun-li,LIU Zhen-zhong,CAI Jing-long..Keratinocyte growth factor phage model peptides can promote epidermal cell proliferation without tumorigenic effect[J].中华医学杂志（英文版）,2010,123(9):1195-1200,6.基金项目
This study was supported by grants from the National Natural Science Foundation of China (No. 30670571 and No. 30772258), Science and Technology Research Program of Shandong Province (No. 2009GG10002078), Scientific Research Development Plan of the Department of Education of Shandong Province(No.J07WD03)and National Basic Research Program of China(973 Program,No.2005CB522603). （No. 30670571 and No. 30772258）
