首页|期刊导航|中华医学杂志（英文版）|In vitro evaluation of the compatibility of a novel collagen-heparan sulfate biological scaffold with olfactory ensheathing cells
In vitro evaluation of the compatibility of a novel collagen-heparan sulfate biological scaffold with olfactory ensheathing cells
TANG Zhou-ping YANG Jie PAN Deng-ji LIU Na LI Zai-wang XIE Xue-wei CHEN Yun SHI Yuan-hong ZENG Wen-gao WANG Shu-xin CHEN Juan
中华医学杂志（英文版）2010，Vol.123Issue(10)：1299-1304,6.
中华医学杂志（英文版）2010，Vol.123Issue(10)：1299-1304,6.DOI:10.3760/cma.j.issn.0366-6999.2010.10.014
In vitro evaluation of the compatibility of a novel collagen-heparan sulfate biological scaffold with olfactory ensheathing cells
In vitro evaluation of the compatibility of a novel collagen-heparan sulfate biological scaffold with olfactory ensheathing cells
摘要
Abstract
Background Stroke and traumatic injury to the nerve system may trigger axonal destruction and the formation of scar tissue, cystic cavitations and physical gaps.Olfactory ensheathing cells (OECs) can secrete neurotrophic factors to promote neurite growth and thus act as a prime candidate for autologous transplantation.Biological scaffolds can provide a robust delivery vehicle to injured nerve tissue for neural cell transplantation strategies, owing to the porous three-dimensional structures (3D).So transplantation of the purposeful cells seeded scaffolds may be a promising method for nerve tissue repair.This study aimed to evaluate the compatibility of a novel collagen-heparan sulfate biological scaffold with olfactory ensheathing cells in vitro.Methods Collagen-heparan sulfate (CHS) biological scaffolds were made, and then the scaffolds and OECs were co-cultured in vitro.The viability of OECs was tested by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium (MTT) assay at days 1, 3, 5 and 7.Statistical analysis was evaluated by student's t test.Significance was accepted at P ＜0.05.OECs were labeled with carboxyfluorescein diacetate succinimidyl ester (CFSE), and the CFSE-labeled OECs were seeded into CHS scaffolds.The attachment and growth of OECs in CHS scaffolds were observed and traced directly by fluorescent microscopy and environmental scanning electron microscope (ESEM).Results CHS biological scaffolds had steady porous 3D structures and no cytotoxicity to OECs (F=0.14, P=0.9330).CHS biological scaffolds were good bridging materials for OECs attachment and proliferation, and they promoted the axonal growth.Conclusion The compatibility of CHS biological scaffolds with OECs is pretty good and CHS biological scaffold is a promising cell carrier for the implantation of OECs in nerve tissue bioengineering.关键词
collagen-heparan sulfate/biological scaffold/olfactory ensheathing cells/compatibilityKey words
collagen-heparan sulfate/biological scaffold/olfactory ensheathing cells/compatibility引用本文复制引用
TANG Zhou-ping,YANG Jie ,PAN Deng-ji,LIU Na,LI Zai-wang,XIE Xue-wei,CHEN Yun,SHI Yuan-hong,ZENG Wen-gao,WANG Shu-xin,CHEN Juan..In vitro evaluation of the compatibility of a novel collagen-heparan sulfate biological scaffold with olfactory ensheathing cells [J].中华医学杂志（英文版）,2010,123(10):1299-1304,6.基金项目
This study was supported by grants from the National Natural Science Foundation of China (No.30570628 and No.30770751). （No.30570628 and No.30770751）
