国际医药卫生导报2010，Vol.16Issue(2)：192-196,5.DOI:10.3760/cma.j.issn.1007-1245.2010.02.023
HPLC测定鼻炎灵滴鼻液中盐酸萘甲唑林和马来酸氯苯那敏含量的方法研究
Determination of Naphazoline Hydrochloride and Chlorphenamine Maleateide in rhinitis nasal drops by HPLC
李国成 1刘春霞 1余晓霞 1林晓珊1
作者信息
- 1. 510120,中山大学附属第二医院药学部
- 折叠
摘要
Abstract
Objective To develop an HPLC assay for the determination of naphazoline hydro-chloride ( NPH ) and Chlorphenamine Maleateide ( CPM ) in rhinitis nasal drops. Methods The RP-HPLC and UV detection . Column for Diamonsil C18 ( 250 mm ×4. 6 mm, 5 μm ) .The mobile phase consisted of 20% 0.05 mol/L Potassium dihydrogen phosphate( 20% Triethylamine ,pH 5. 0, adjusted by phosphate solution ), 60% methanol and 20% water. The flow rate was 0.8ml /min. Injection volume 5μL-Column temperature 40℃ , Column pressure 75bar, Detection wavelength λ = 225 nm. Results The retention times for NPH and CPM were 4.758 and 7.364min, respectively. The calibration curveswere linear in the range of 6.02 ～ 72.24 μg/mL for NPH and 6.08～72.96 μg/ml for CPM. The average recoveries for NPH and CPM were 97.50% ( RSD 0.37% ) and 97.24% ( RSD 0.69 % ) , respectively. Conclusion This simple, accurate and selective method is suitable for the quality control of rhinitis nasal drops.关键词
高效液相色谱法/盐酸萘甲唑林/马来酸氯苯那敏Key words
HPLC/Naphazoline Hydrochloride/Chlorphenamine Maleateide引用本文复制引用
李国成,刘春霞,余晓霞,林晓珊..HPLC测定鼻炎灵滴鼻液中盐酸萘甲唑林和马来酸氯苯那敏含量的方法研究[J].国际医药卫生导报,2010,16(2):192-196,5.
