植物研究2008，Vol.28Issue(6)：710-715,6.
番茄泛素蛋白基因LeEBF1和LeEBF2的克隆表达分析
Molecular Cloning and Characterization of LeEBF1 and LeEBF2 from Tomato
摘要
Abstract
Two full-length cDNAs of LeEBF1 (EIN3 binding F-box protein 1)and LeEBF2(EIN3 bind-ing F-box protein 2)were cloned from tomato (Solanum lycopersicum) by RT-PCR and rapid amplifica-tion of eDNA ends (RACE). They were 2 866 and 2 891 bp, with open reading frame (ORF) of 1 911 bp and 1 995 bp, encoding 637 and 665 amino acids, respectively. Putative LeEBF1 and LeEBF2 pro-teins, each containing a well-conserved F-box motif in the amino terminus and 14 tandem leucine-rich repeats (LRRs) in the carboxyl terminus, shared 58.6% of identity with each other. Identities with other species EBF proteins within F box motif ranged from 24.4% to 73.2%. Northern blot analysis re-sults indicated that the expression levels of LeEBF1 and LeEBF2 in young leaves were higher than those in mature leaves of wild type and Nr. During the development of fruits, LeEBF1 and LeEBF2 expressed relatively weaker at IMG stage than at other stages tested. In a conclusion, LeEBF1 and LeEBF2 may play an important role in leaf and fruit development of tomato.关键词
LeEBF1/LeEBF2/番茄/泛素途径Key words
LeEBF1/LeEBF2/Tomato/UbiquitinationAbbreviations DPA, days post anthesis/LRRs, leucine-ricb repeats/Nr, Never-ripening/ORF,open reading frame/RACE, rapid amplification of cDNA ends/rin, ripening inhibitor/UPS, ubiquitin proteasome system分类
农业科技引用本文复制引用
龙皎月,胡宗利,陈绪清,严波,陈国平..番茄泛素蛋白基因LeEBF1和LeEBF2的克隆表达分析[J].植物研究,2008,28(6):710-715,6.基金项目
Supported by National Natural Science Foundation of China (No. 30600044) and Natural Science Foundation of Chongqing ofChina (No. cstc, 2006BB1193,2006BA5006). （No. 30600044）
