生殖医学杂志2008，Vol.17Issue(z1)：26-32,7.DOI:10.3969/j.issn.1004-3845.2008.z1.006
Effect of washing mineral oil on development of mouse embryos in vitro and in vivo after embryo transfer
Effect of washing mineral oil on development of mouse embryos in vitro and in vivo after embryo transfer
摘要
Abstract
Objective:To establish a simple and effective washing procedure for both used and purchased mineral oil,that can be used for embryo culture.Methods:A complete test system has been used for this purpose.There are 3 steps in our new washing proto-col.First,the oil was mixed with 95% ethanol at 1:1,the bottle being shaken by hand for 10 minutes,then sepa-rated.Second,the oil was heated to boiling point with 0.31 mol/L NaCl for 30 minutes.Third,anhydrous Na:SO4 was put into the oil for further treatment.1-cell stage embryos of a KM strain mouse have been collected surgically and cultured.Cleavage and blastocyst stage development were recorded and some embryos were transferred into re-cipients.Results:The results show that recycled oil can promote the development from 2-cell to blastocyst stage(23.3%)when compared with that of control(16.9%).Offspring have been obtained at 44 %(7/16),16 %(3/19)from washed recycled oil and control oil respectively.Conclusion:This washing procedure is safe and effective for the used treatment and for other sources of mineral oil used for embryo culture.关键词
KM strain mouse/1-cell stage embryo/Mineral oil/Washing system/Embryo culture/Embryo transferKey words
KM strain mouse/1-cell stage embryo/Mineral oil/Washing system/Embryo culture/Embryo transfer引用本文复制引用
Li Hui,Zhang Li-xuan,Zhong Yu,Zhu Kai,Zhang Tian,Wang Min-kang..Effect of washing mineral oil on development of mouse embryos in vitro and in vivo after embryo transfer[J].生殖医学杂志,2008,17(z1):26-32,7.基金项目
This research was supported by China NNSF(No. 30560056),the Scientific and Technical Department of Yunnan Porovince(2005PY 01), and the Educational Department of Yunnan Province(06Y1168) （No. 30560056）
