老年心脏病学杂志（英文版）2008，Vol.5Issue(4)：243-247,5.
Effects of sea anemone toxin anthopleurin-Q on potassium currents in rats and guinea pig ventricular myocytes
Effects of sea anemone toxin anthopleurin-Q on potassium currents in rats and guinea pig ventricular myocytes
Yan Huang 1Emmy Okello 2Yang Li 3Hasimu Buaijiaer 1Juan Sun 1Ping Fan 1Jianguo Song 1Yuemei Hou 1Mingjiang Li 2Salim Mohamed2
作者信息
- 1. Cardiac Department,Xinjiang Medical University First Affiliated Hospital Urumqi 830054,China
- 2. Division of Cardiology,Renmin Hospital Wuhan University,Wuhan 430060,China
- 3. Institute of Geriatric Cardiology of Chinese PLA General Hospital Beijing 100853,China
- 折叠
摘要
Abstract
To investigate the effect of sea anemone toxin anthopleurin-Q (AP-Q) on potassium currents in isolated rats and guinea pig ventricular myocytes.Methods The ventricular cells of guinea pigs and SD rats were obtained by enzymatic dissociation method.Whole cell patch clamp technique was used to record potassium currents (Ito,IK,and IK1).Results AP-Q 3-100 nmol/L increased Ito in a concentration-dependent manner,with an EC50 value of 12.7 nmol/L.At a potential of +50mV,AP-Q 10nmol/L increased Ito from (13.3±3.4) pA pF-1 to (19.46±4.3) pA pF-1.AP-Q 0.1-100 nmol/L increased IK and IK tail in a concentration-dependent manner with EC50 values of 4.7 nmol/L and 5.0 nmol/L,respectively.AP-Q 1 pmol/L-100 nmol/L increased IK1 in dose-dependent manner,with an EC50 of 0.2 nmol/L.Conclusions The effect of AP-Q on Ito,IK and IK1 may partly explain its mechanism in shortening APD and increasing RP.(J Geriatr Cardiol 2008;5:243-247)关键词
sea anemone toxin AP-Q/ventricular myocytes/potassium currentKey words
sea anemone toxin AP-Q/ventricular myocytes/potassium current引用本文复制引用
Yan Huang,Emmy Okello,Yang Li,Hasimu Buaijiaer,Juan Sun,Ping Fan,Jianguo Song,Yuemei Hou,Mingjiang Li,Salim Mohamed..Effects of sea anemone toxin anthopleurin-Q on potassium currents in rats and guinea pig ventricular myocytes[J].老年心脏病学杂志（英文版）,2008,5(4):243-247,5.
