中国中西医结合杂志2011，Vol.31Issue(1)：23-27,5.
祛痰化瘀解毒方药对高脂血症痰瘀互阻证患者单核细胞亚群异常的影响
Impact of Qutan Huayu Jiedu Herbs on Monocyte Subpopulations Abnormality in Patients with Hyperlipidemia of Phlegm-Stagnancy Obstruction Syndrome Pattern
摘要
Abstract
Objective To investigate the distribution of monocyte subpopulations and the changes of Tolllike receptor 4 (TLR4), CD163 and CD36 expressions in the peripheral blood of patients with hyperlipidemia of phlegm-stagnancy obstruction syndrome pattem( HLE-PSO), and to evaluate the intervening effects of Qutan Huayu Jiedu (QHJ) herbs on these parameters. Methods Monocytes in the peripheral blood were sorted using flow cytometry into 3 subpopulations: the CD14high CD16-( Mo1 ), the CD14high CD16+ (Mo2a) and the CD14low CD16+(Mo2b) subpopulation. The percentages of various monocyte subpopulations in 83 patients and 42 matched healthy controls were determined and the levels of their surface receptors TLR4, CD163 and CD36 expressions were assayed with flow cytometer. Furthermore, patients allocated in the tested group (10 patients) and the control group (11 patients) were treated with QHJ Herbs and Qutan Huayu (QH, removing Phlegm and dissolving stagnancy but without detoxication) herbs respectively. The changes of monocyte subpopulations percentage and TLR4, CD163 and CD36 expressions were determined 4 weeks after they received treatment. Results Percentage of Mo2a subpopulation was significantly higher in HLE-PSO patients than the normal range (5. 35 ± 2. 57 vs. 3. 09 ± 2. 38,P＜0. 01 ), but the deviation of the other two subpopulations in percentage was insignificant. The TLR4 expression on Mo1 monocyte in patients was lower than normal (50. 73 ±24. 45 vs. 69. 92 ±21.06, P＜0. 01 ), while CD163 and CD36 expressions of all three subpopulations in patients were similar to those in healthy persons respectively.After 4 weeks of treatment, a significant lowered Mo2a proportions were observed in the tested group (3. 73 ± 1.05vs. 5. 50 ±2. 06,P=0. 043); but not in the control group (4. 20 ±1.81 vs. 5. 65 ±1.89,P=0. 097), while the levels of TLR4, CD163 and CD36 were not significantly altered after treatment in both groups. Conclusions The elevated proportions of Mo2a subpopulation and the lowered TLR4 expression in Mo1 subpopulation are the characteristic changes in HLE-PSO patients, which might be related with the hyperlipidemia caused immune injury in patients.QHJ herbs could effectively improve the disproportion of Mo2a subpopulation.关键词
高脂血症/痰瘀互阻证/单核细胞亚群/祛痰化瘀解毒方药引用本文复制引用
马雅銮,王宪波,曾辉,王亚红,韩俊燕,郝禹,陈冰,宋剑南,韩俊阁,尚涛,徐丽丽..祛痰化瘀解毒方药对高脂血症痰瘀互阻证患者单核细胞亚群异常的影响[J].中国中西医结合杂志,2011,31(1):23-27,5.基金项目
北京市自然科学基金资助项目(No.7102119) （No.7102119）
中国中医科学院自主选题研究项目(No.ZZ-20006008) （No.ZZ-20006008）
