植物学报2011，Vol.46Issue(1)：1-10,10.DOI:10.3724/SP.J.1259.2011.00001
银杏叶绿体ndhF RNA编辑现象分析及C290位编辑对胁迫处理的响应
Analysis of Editing Sites for Chloroplast ndhF in Ginkgo biloba and the Editing Efficiency at C290 in Response to Different Stresses
摘要
Abstract
RNA editing is a post-transcriptional modification process including nucleotide insertion, deletion or replacement. Mostly, RNA editing converts amino acid from hydrophilic to hydrophobic and restores conservation of homologous amino acid residues among different species. In this paper, we analyzed RNA editing sites of ndhF gene of chloroplast in Ginkgo biloba by PCR and RT-PCR. The results show that total 21 editing sites are found in this gene. All 21 sites are partially edited with C to U conversions. Among of 21 editing sites, C290 is probably a critical editing site for ndhF to form adapted secondary and tertiary structure by using bioinformatics analysis. We further examined ndhF C290 editing eftciency with enzyme digestion method under low or high temperature, salt, drought and darkness treatment. The results show that ndhF C290 editing efficiency is significantly affected by temperature and darkness than by salt and drought treatment.关键词
编辑效率/银杏/ndhF基因/RNA编辑/胁迫处理引用本文复制引用
马艳莉,陈海燕,王继刚,俞嘉宁..银杏叶绿体ndhF RNA编辑现象分析及C290位编辑对胁迫处理的响应[J].植物学报,2011,46(1):1-10,10.基金项目
陕西师范大学中央高校基本科研业务费(No.2010-2012,No.GK200902028)和国家大学生创新性试验计划(No.081071810) （No.2010-2012,No.GK200902028）
