中国中西医结合杂志2011，Vol.31Issue(1)：58-61,4.
胡黄连苷Ⅱ对大鼠脑缺血再灌注损伤后TLR4及NFκB表达的影响
Effect of Picroside Ⅱ on Expressions of TLR4 and NFκB in Rats with Cerebral Ischemia Reperfusion Injury
摘要
Abstract
Objective To explore the effects of picrodide Ⅱ on the expressions of Toll-like receptor 4 (TLR4) and nuclear transcription factor κB (NFκB) in brain tissue of rat after cerebral ischemic reperfusion (I/R) injury. Methods Ten rats from 60 adult healthy female Wister rats received sham-operation were set as the sham-operative group. Established as middle crerebral I/R model (MCAO/R) by thread tying method, the 30 successfully modeled rats were equally randomized into the negative control group, the positive control group and the treatment group. Besides, rats in the treatment group and the poshtive control group were respectively intervened with picrodide Ⅱ (10 mg/kg) and salvianic acid A sodium (10 mg/kg) via caudal vein injection before I/R injury, while rats in the sham-operative group and the negative group were injected with equal volume of 0.1 mol/L PBS. Immunohistochemistry stain was used to determine the expressions of TLR4 and NFκB, and the apoptotic cells were counted by TUNEL-immunofluorescence assay. Results In the sham-operative group, the TLR4 and NFκB expressed weakly with few TUNEL positive cells scattering in the cortex, striatum and hippocampus. As compared with the sham-operative group, TLR4 and NFκB in the negative control group were significantly higher both in absorption A) value and cell number ( P＜0. 05). In the treatment group and the positive control group, the expressions of TLR4 and NFκB and the number of TUNEL positive cells were significantly lower than those in the negative control group ( P ＜0. 05), but no significant difference was shown between the two treated groups (P＞0.05). Conclusions Picroside Ⅱ could down-regulate the expressions of TLR4 and NFκB, and inhibit the inflammatory response induced apoptosis in cerebral I/R injured rats.关键词
胡黄连苷 Ⅱ/脑缺血再灌注/Toll 样受体 4/核转录因子 κB/细胞凋亡/大鼠引用本文复制引用
郭云良,沈卫,杜芳,李琴,李震..胡黄连苷Ⅱ对大鼠脑缺血再灌注损伤后TLR4及NFκB表达的影响[J].中国中西医结合杂志,2011,31(1):58-61,4.基金项目
国家自然科学基金资助项目(No.30873391,No.81041092) （No.30873391,No.81041092）
