工业微生物2011，Vol.41Issue(2)：37-42,6.DOI:10.3969/j.issn.1001-6678.2011.02.008
丙酮酸钠对E.coli CCTCC M208088发酵生产聚唾液酸的影响
Effects of sodium pyruvate on synthesis of polysialic acid by E.coli CCTCC M208088
摘要
Abstract
Effects of sodium pyruvate on the biosynthesis of polysialic acid by E. coli CCTCC M208088 were investigated . The results showed that the addition of sodium pyruvate enhanced the production of polysialic acid , and the optimum time and concentration for sodium pyruvate addition were 0h and 6g/L . At the end of fermentation, the polysialic acid production reached 3 .47 g/L , increased by 73％ as compared with the control , and the conversion rate increased by 125％. The final polysialic acid production was as high as 6 .6g/L in a 30 L reactor , increased by 53 .5％ as compared with the control. The main role of sodium pyruvate was to enhance TCA cycle of the bacteria in vivo and probably improved the level of intracellular energy charge , which would stimulate polysialic acid synthesis .关键词
聚唾液酸/丙酮酸钠/能荷/有机酸引用本文复制引用
申凤丹,刘金龙,吴剑荣,詹晓北..丙酮酸钠对E.coli CCTCC M208088发酵生产聚唾液酸的影响[J].工业微生物,2011,41(2):37-42,6.基金项目
国家“863”计划项目(“针对微生物多糖高粘度发酵的关键技术与设备”，2006AA02Z207)资助. （“针对微生物多糖高粘度发酵的关键技术与设备”，2006AA02Z207）
