工业微生物2011，Vol.41Issue(2)：1-6,6.DOI:10.3969/j.issn.1001-6678.2011.02.001
头孢菌素C去乙酰基酶产生菌的筛选、酶学性质及产酶条件优化
Screening and identification of CAH-producing strain,enzyme properties of cephalosporin-C deacetylase and optimization of enzyme production
摘要
Abstract
When 7-aminocephalosporanic acid (7-ACA) was used as a single carbon source in the enrichment culture medium for screening 7-ACA-degrading microorganisms , a strain producing CAH with high yield was obtained . It was identified as red yeast . The enzyme was most active at 25℃ and pH 7 .0. The enzyme was stable at temperatures lower than 40 ℃ and within pH 5 .5～8 .0 . The optimized conditions for enzyme production were as followings : glucose 30 g/L , homemade yeast 20 g/L and KH2 PO4 5 g/L , while the initial medium pH was 7 .0 .关键词
7-氨基头孢烷酸/去乙酰基-7-氨基头孢烷酸/头孢菌素C乙酰水解酶引用本文复制引用
韩云宾,周文瑜,胡媛,魏东芝,沈亚领,方善综,金雄熊,叶凤起..头孢菌素C去乙酰基酶产生菌的筛选、酶学性质及产酶条件优化[J].工业微生物,2011,41(2):1-6,6.基金项目
上海市重点学科建设项目资助(B505). （B505）
